Age- and Sex-Related Characteristics of Tonic Gaba Currents in the Rat Substantia Nigra Pars Reticulata

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F15%3A10294505" target="_blank" >RIV/00216208:11130/15:10294505 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/15:10294505

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11064-015-1523-3" target="_blank" >http://dx.doi.org/10.1007/s11064-015-1523-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11064-015-1523-3" target="_blank" >10.1007/s11064-015-1523-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Age- and Sex-Related Characteristics of Tonic Gaba Currents in the Rat Substantia Nigra Pars Reticulata

Popis výsledku v původním jazyce

Previous studies have shown that the pharmacologic effects of GABAergic drugs and the postsynaptic phasic GABA(A)ergic inhibitory responses in the anterior part of the rat substantia nigra pars reticulata (SNRA) are age- and sex-specific. Here, we investigate whether there are age- and sex-related differences in the expression of the delta GABA(A) receptor (GABA(A)R) subunit and GABA(A)R mediated tonic currents. We have used delta-specific immunochemistry and whole cell patch clamp to study GABA(A)R mediated tonic currents in the SNRA of male and female postnatal day (PN) PN5-9, PN11-16, and PN25-32 rats. We observed age-related decline, but no sex-specific changes, in bicuculline (BIM) sensitive GABA(A)R tonic current density, which correlated with the decline in delta subunit in the SNRA between PN15 and 30. Furthermore, we show that the GABA(A)R tonic currents can be modified by muscimol (GABA(A)R agonist; partial GABA(C)R agonist), THIP (4,5,6,7-tetrahydroisoxazolo (5,4-c)pyridin-3

Název v anglickém jazyce

Age- and Sex-Related Characteristics of Tonic Gaba Currents in the Rat Substantia Nigra Pars Reticulata

Popis výsledku anglicky

Previous studies have shown that the pharmacologic effects of GABAergic drugs and the postsynaptic phasic GABA(A)ergic inhibitory responses in the anterior part of the rat substantia nigra pars reticulata (SNRA) are age- and sex-specific. Here, we investigate whether there are age- and sex-related differences in the expression of the delta GABA(A) receptor (GABA(A)R) subunit and GABA(A)R mediated tonic currents. We have used delta-specific immunochemistry and whole cell patch clamp to study GABA(A)R mediated tonic currents in the SNRA of male and female postnatal day (PN) PN5-9, PN11-16, and PN25-32 rats. We observed age-related decline, but no sex-specific changes, in bicuculline (BIM) sensitive GABA(A)R tonic current density, which correlated with the decline in delta subunit in the SNRA between PN15 and 30. Furthermore, we show that the GABA(A)R tonic currents can be modified by muscimol (GABA(A)R agonist; partial GABA(C)R agonist), THIP (4,5,6,7-tetrahydroisoxazolo (5,4-c)pyridin-3

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

—

Projekt

<a href="/cs/project/GD309%2F08%2FH079" target="_blank" >GD309/08/H079: Doktorský studijní projekt v oboru neurovědy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neurochemical Research

ISSN

0364-3190

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

747-757

Kód UT WoS článku

000351861100012

EID výsledku v databázi Scopus

2-s2.0-84939975788