Recurrent deletions of IKZF1 in pediatric acute myeloid leukemia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F15%3A10315710" target="_blank" >RIV/00216208:11130/15:10315710 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/15:10315710

Výsledek na webu

<a href="http://dx.doi.org/10.3324/haematol.2015.124321" target="_blank" >http://dx.doi.org/10.3324/haematol.2015.124321</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3324/haematol.2015.124321" target="_blank" >10.3324/haematol.2015.124321</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Recurrent deletions of IKZF1 in pediatric acute myeloid leukemia

Popis výsledku v původním jazyce

IKAROS family zinc finger 1/IKZF1 is a transcription factor important in lymphoid differentiation, and a known tumor suppressor in acute lymphoid leukemia. Recent studies suggest that IKZF1 is also involved in myeloid differentiation. To investigate whether IKZF1 deletions also play a role in pediatric acute myeloid leukemia, we screened a panel of pediatric acute myeloid leukemia samples for deletions of the IKZF1 locus using multiplex ligation-dependent probe amplification and for mutations using direct sequencing. Three patients were identified with a single amino acid variant without change of IKZF1 length. No frame-shift mutations were found. Out of 11 patients with an IKZF1 deletion, 8 samples revealed a complete loss of chromosome 7, and 3 casesa focal deletion of 0.10.9Mb. These deletions included the complete IKZF1 gene (n=2) or exons 14 (n=1), all leading to a loss of IKZF1 function. Interestingly, differentially expressed genes in monosomy 7 cases (n=8) when compared to non

Název v anglickém jazyce

Recurrent deletions of IKZF1 in pediatric acute myeloid leukemia

Popis výsledku anglicky

IKAROS family zinc finger 1/IKZF1 is a transcription factor important in lymphoid differentiation, and a known tumor suppressor in acute lymphoid leukemia. Recent studies suggest that IKZF1 is also involved in myeloid differentiation. To investigate whether IKZF1 deletions also play a role in pediatric acute myeloid leukemia, we screened a panel of pediatric acute myeloid leukemia samples for deletions of the IKZF1 locus using multiplex ligation-dependent probe amplification and for mutations using direct sequencing. Three patients were identified with a single amino acid variant without change of IKZF1 length. No frame-shift mutations were found. Out of 11 patients with an IKZF1 deletion, 8 samples revealed a complete loss of chromosome 7, and 3 casesa focal deletion of 0.10.9Mb. These deletions included the complete IKZF1 gene (n=2) or exons 14 (n=1), all leading to a loss of IKZF1 function. Interestingly, differentially expressed genes in monosomy 7 cases (n=8) when compared to non

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Haematologica

ISSN

0390-6078

e-ISSN

—

Svazek periodika

100

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

9

Strana od-do

1151-1159

Kód UT WoS článku

000365081500025

EID výsledku v databázi Scopus

2-s2.0-84940949154