Chronic hypoxia increases fetoplacental vascular resistance in rat placenta perfused with blood
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F16%3A10332612" target="_blank" >RIV/00216208:11130/16:10332612 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.4149/BLL_2016_113" target="_blank" >http://dx.doi.org/10.4149/BLL_2016_113</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4149/BLL_2016_113" target="_blank" >10.4149/BLL_2016_113</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Chronic hypoxia increases fetoplacental vascular resistance in rat placenta perfused with blood
Popis výsledku v původním jazyce
BACKGROUND: Chronic hypoxia elevates vascular resistance on the fetal side of the placenta. However, when a low-viscosity perfusate is used, the increase in resistance caused by chronic hypoxia is relatively small (12 mmHg). In the present study, we tested the hypothesis that perfusion with more viscous fluid (blood) will reveal more substantial effect of chronic hypoxia. METHODS: Using an isolated, dually perfused rat placenta perfused at a constant flow rate with homologous blood, perfusion pressure on the fetal side was measured. Then, the relationship between perfusion pressure and flow (P/Q) was determined. RESULTS: Fetoplacental vascular resistance was increased by chronic hypoxia (10 % O-2) during the last third of gestation. This was observed when the placentas were perfused with a low viscosity Krebs solution and more enhanced when perfused with blood. Nevertheless, we found no clear advantage for using blood instead of Krebs solution to study the effects of hypoxia on the fetoplacental vasculature. The elevation of fetoplacental vascular resistance caused by chronic hypoxia was at least partly resistant to acute reoxygenation. CONCLUSION: Using blood for the perfusion of the isolated rat placenta does not confer any clear methodological advantage over using Krebs solution (Tab. 2, Fig. 2, Ref. 21). Text in PDF www.elis.sk.
Název v anglickém jazyce
Chronic hypoxia increases fetoplacental vascular resistance in rat placenta perfused with blood
Popis výsledku anglicky
BACKGROUND: Chronic hypoxia elevates vascular resistance on the fetal side of the placenta. However, when a low-viscosity perfusate is used, the increase in resistance caused by chronic hypoxia is relatively small (12 mmHg). In the present study, we tested the hypothesis that perfusion with more viscous fluid (blood) will reveal more substantial effect of chronic hypoxia. METHODS: Using an isolated, dually perfused rat placenta perfused at a constant flow rate with homologous blood, perfusion pressure on the fetal side was measured. Then, the relationship between perfusion pressure and flow (P/Q) was determined. RESULTS: Fetoplacental vascular resistance was increased by chronic hypoxia (10 % O-2) during the last third of gestation. This was observed when the placentas were perfused with a low viscosity Krebs solution and more enhanced when perfused with blood. Nevertheless, we found no clear advantage for using blood instead of Krebs solution to study the effects of hypoxia on the fetoplacental vasculature. The elevation of fetoplacental vascular resistance caused by chronic hypoxia was at least partly resistant to acute reoxygenation. CONCLUSION: Using blood for the perfusion of the isolated rat placenta does not confer any clear methodological advantage over using Krebs solution (Tab. 2, Fig. 2, Ref. 21). Text in PDF www.elis.sk.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FA - Kardiovaskulární nemoci včetně kardiochirurgie
OECD FORD obor
—
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Bratislavské lekárske listy
ISSN
0006-9248
e-ISSN
—
Svazek periodika
117
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
SK - Slovenská republika
Počet stran výsledku
4
Strana od-do
583-586
Kód UT WoS článku
000387631100006
EID výsledku v databázi Scopus
2-s2.0-85014922999