Apoferritin as an ubiquitous nanocarrier with excellent shelf life

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10360756" target="_blank" >RIV/00216208:11130/17:10360756 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/17:10360756 RIV/00064203:_____/17:10360756

Výsledek na webu

<a href="http://dx.doi.org/10.2147/IJN.S130267" target="_blank" >http://dx.doi.org/10.2147/IJN.S130267</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S130267" target="_blank" >10.2147/IJN.S130267</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Apoferritin as an ubiquitous nanocarrier with excellent shelf life

Popis výsledku v původním jazyce

Due to many adverse effects of conventional chemotherapy, novel methods of targeting drugs to cancer cells are being investigated. Nanosize carriers are a suitable platform for this specific delivery. Herein, we evaluated the long-term stability of the naturally found protein nanocarrier apoferritin (Apo) with encapsulated doxorubicin (Dox). The encapsulation was performed using Apo&apos;s ability to disassemble reversibly into its subunits at low pH (2.7) and reassemble in neutral pH (7.2), physically entrapping drug molecules in its cavity (creating ApoDox). In this study, ApoDox was prepared in water and phosphate-buffered saline and stored for 12 weeks in various conditions (-20 degrees C, 4 degrees C, 20 degrees C, and 37 degrees C in dark, and 4 degrees C and 20 degrees C under ambient light). During storage, a very low amount of prematurely released drug molecules were detected (maximum of 7.5% for ApoDox prepared in PBS and 4.4% for ApoDox prepared in water). Fourier-transform infrared spectra revealed no significant differences in any of the samples after storage. Most of the ApoDox prepared in phosphate-buffered saline and ApoDox prepared in water and stored at -20 degrees C formed very large aggregates (up to 487% of original size). Only ApoDox prepared in water and stored at 4 degrees C showed no significant increase in size or shape. Although this storage caused slower internalization to LNCaP prostate cancer cells, ApoDox (2.5 mu M of Dox) still retained its ability to inhibit completely the growth of 1.5x10(4) LNCaP cells after 72 hours. ApoDox stored at 20 degrees C and 37 degrees C in water was not able to deliver Dox inside the nucleus, and thus did not inhibit the growth of the LNCaP cells. Overall, our study demonstrates that ApoDox has very good stability over the course of 12 weeks when stored properly (at 4 degrees C), and is thus suitable for use as a nanocarrier in the specific delivery of anticancer drugs to patients.

Název v anglickém jazyce

Apoferritin as an ubiquitous nanocarrier with excellent shelf life

Popis výsledku anglicky

Due to many adverse effects of conventional chemotherapy, novel methods of targeting drugs to cancer cells are being investigated. Nanosize carriers are a suitable platform for this specific delivery. Herein, we evaluated the long-term stability of the naturally found protein nanocarrier apoferritin (Apo) with encapsulated doxorubicin (Dox). The encapsulation was performed using Apo&apos;s ability to disassemble reversibly into its subunits at low pH (2.7) and reassemble in neutral pH (7.2), physically entrapping drug molecules in its cavity (creating ApoDox). In this study, ApoDox was prepared in water and phosphate-buffered saline and stored for 12 weeks in various conditions (-20 degrees C, 4 degrees C, 20 degrees C, and 37 degrees C in dark, and 4 degrees C and 20 degrees C under ambient light). During storage, a very low amount of prematurely released drug molecules were detected (maximum of 7.5% for ApoDox prepared in PBS and 4.4% for ApoDox prepared in water). Fourier-transform infrared spectra revealed no significant differences in any of the samples after storage. Most of the ApoDox prepared in phosphate-buffered saline and ApoDox prepared in water and stored at -20 degrees C formed very large aggregates (up to 487% of original size). Only ApoDox prepared in water and stored at 4 degrees C showed no significant increase in size or shape. Although this storage caused slower internalization to LNCaP prostate cancer cells, ApoDox (2.5 mu M of Dox) still retained its ability to inhibit completely the growth of 1.5x10(4) LNCaP cells after 72 hours. ApoDox stored at 20 degrees C and 37 degrees C in water was not able to deliver Dox inside the nucleus, and thus did not inhibit the growth of the LNCaP cells. Overall, our study demonstrates that ApoDox has very good stability over the course of 12 weeks when stored properly (at 4 degrees C), and is thus suitable for use as a nanocarrier in the specific delivery of anticancer drugs to patients.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

<a href="/cs/project/GA17-12816S" target="_blank" >GA17-12816S: Konstrukce modifikovaných apoferitinových nanočástic s protinádorovými léčivy a studium mechanismů potencujících jejich efektivitu v terapii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Nanomedicine [online]

ISSN

1178-2013

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

NZ - Nový Zéland

Počet stran výsledku

14

Strana od-do

2265-2278

Kód UT WoS článku

000397274500001

EID výsledku v databázi Scopus

2-s2.0-85016330513