Analysis of circulating miRNAs in patients with familial hypercholesterolaemia treated by LDL/Lp(a) apheresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10365939" target="_blank" >RIV/00216208:11130/17:10365939 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/17:10365939 RIV/00179906:_____/17:10365939 RIV/00064203:_____/17:10365939 RIV/00023001:_____/17:00076209

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S156756881730079X?via%3Dihub" target="_blank" >http://www.sciencedirect.com/science/article/pii/S156756881730079X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.atherosclerosissup.2017.05.037" target="_blank" >10.1016/j.atherosclerosissup.2017.05.037</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of circulating miRNAs in patients with familial hypercholesterolaemia treated by LDL/Lp(a) apheresis

Popis výsledku v původním jazyce

Background: LDL/Lp(a) apheresis therapy is a well-established method of aggressively lowering LDL and Lp(a). Recently, miRNAs have been discussed as markers of vascular status including atherosclerosis. MiRNAs inhibit post-transcriptional processes through RNA duplex formation resulting in gene silencing or regulation of gene expression. Materials and methods: We measured a profile of 175 plasma-circulating miRNAs using pre-defined Serum/Plasma Focus Human microRNA PCR Panels in pooled samples of 11 subjects with familial hypercholesterolaemia under long-term apheresis treatment. Subsequently we analysed expressions of ten pre-selected miRNAs potentially involved in lipid homeostasis in the same group of subjects. We compared plasma-circulating miRNA levels isolated from peripheral blood collected immediately before and after apheresis. Results: The greatest differences in plasma levels were found in miR-451a, miR-16, miR-19a/b, miR-223 and miR-185. In subsequent individual miRNA assay we detected a significant increase in miR-33b levels after apheresis (P &lt; 0.05). Additionally, correlations between plasma lipids and miR-33a (P &lt; 0.04) and miR-122 (P &lt; 0.01) have been determined. Moreover, miR-122 levels in LDLR homozygotes were higher compared to heterozygotes after, but not before, apheresis treatment (P &lt; 0.04). Conclusions: LDL/Lp(a) apheresis has an impact on miRNAs associated with lipid homeostasis and vascular status.

Název v anglickém jazyce

Analysis of circulating miRNAs in patients with familial hypercholesterolaemia treated by LDL/Lp(a) apheresis

Popis výsledku anglicky

Background: LDL/Lp(a) apheresis therapy is a well-established method of aggressively lowering LDL and Lp(a). Recently, miRNAs have been discussed as markers of vascular status including atherosclerosis. MiRNAs inhibit post-transcriptional processes through RNA duplex formation resulting in gene silencing or regulation of gene expression. Materials and methods: We measured a profile of 175 plasma-circulating miRNAs using pre-defined Serum/Plasma Focus Human microRNA PCR Panels in pooled samples of 11 subjects with familial hypercholesterolaemia under long-term apheresis treatment. Subsequently we analysed expressions of ten pre-selected miRNAs potentially involved in lipid homeostasis in the same group of subjects. We compared plasma-circulating miRNA levels isolated from peripheral blood collected immediately before and after apheresis. Results: The greatest differences in plasma levels were found in miR-451a, miR-16, miR-19a/b, miR-223 and miR-185. In subsequent individual miRNA assay we detected a significant increase in miR-33b levels after apheresis (P &lt; 0.05). Additionally, correlations between plasma lipids and miR-33a (P &lt; 0.04) and miR-122 (P &lt; 0.01) have been determined. Moreover, miR-122 levels in LDLR homozygotes were higher compared to heterozygotes after, but not before, apheresis treatment (P &lt; 0.04). Conclusions: LDL/Lp(a) apheresis has an impact on miRNAs associated with lipid homeostasis and vascular status.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Atherosclerosis, Supplements

ISSN

1567-5688

e-ISSN

—

Svazek periodika

30

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

7

Strana od-do

128-134

Kód UT WoS článku

000415625000019

EID výsledku v databázi Scopus

2-s2.0-85032457877