Appearance of cytomegalovirus-specific T-cells predicts fast resolution of viremia post hematopoietic stem cell transplantation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10373644" target="_blank" >RIV/00216208:11130/17:10373644 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/17:10373644

Výsledek na webu

<a href="https://doi.org/10.1002/cyto.b.21348" target="_blank" >https://doi.org/10.1002/cyto.b.21348</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cyto.b.21348" target="_blank" >10.1002/cyto.b.21348</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Appearance of cytomegalovirus-specific T-cells predicts fast resolution of viremia post hematopoietic stem cell transplantation

Popis výsledku v původním jazyce

BackgroundCytomegalovirus (CMV) specific T-cells are known to provide long-term control of CMV reactivation, which is a frequent complication of hematopoietic stem cell transplantation. We have studied 58 pediatric patients after hematopoietic stem cell transplantation who suffered from CMV reactivation to reveal which functional T cell subset is best correlating with successful reactivation resolution and which protects from reactivation episode. MethodsDetection of 30 combinatorial subsets of four types of response to ex vivo CMV stimulation (IFN secretion, IL-2 secretion, CD40L upregulation and degranulation) that were detectable on either CD8+ or CD4+ T cells through flow cytometry intracellular cytokine staining was used. ResultsWe found that the presence of CD8+ dual positive (IFN+ and IL-2+) cells is the most accurate functional parameter that can predict fast resolution of CMV reactivation. Next, we show that the presence of CD8+ dual positive (IFN+ and IL-2+) and CD8+ IFN+ cells provides a protective effect (a hazard risk of 0.28 (confidence interval 0.18 - 0.43) and 0.45 (CI 0.27 - 0.75), respectively) and the presence of corticotherapy increases the risk of reactivation (HR 2.47 (CI 1.82-3.36)). Thus, a patient without corticotherapy and with both of the critical T cell subsets present has a cumulative 19.6 times lower risk of developing CMV reactivation than a patient on corticotherapy and without CD8+ dual positive (IFN+ and IL-2+) or CD8+ IFN+ cells. ConclusionsWe have established parameters of CMV specific functional response ex vivo that can be used in assisting clinical management of patients with CMV reactivation. (c) 2015 International Clinical Cytometry Society

Název v anglickém jazyce

Appearance of cytomegalovirus-specific T-cells predicts fast resolution of viremia post hematopoietic stem cell transplantation

Popis výsledku anglicky

BackgroundCytomegalovirus (CMV) specific T-cells are known to provide long-term control of CMV reactivation, which is a frequent complication of hematopoietic stem cell transplantation. We have studied 58 pediatric patients after hematopoietic stem cell transplantation who suffered from CMV reactivation to reveal which functional T cell subset is best correlating with successful reactivation resolution and which protects from reactivation episode. MethodsDetection of 30 combinatorial subsets of four types of response to ex vivo CMV stimulation (IFN secretion, IL-2 secretion, CD40L upregulation and degranulation) that were detectable on either CD8+ or CD4+ T cells through flow cytometry intracellular cytokine staining was used. ResultsWe found that the presence of CD8+ dual positive (IFN+ and IL-2+) cells is the most accurate functional parameter that can predict fast resolution of CMV reactivation. Next, we show that the presence of CD8+ dual positive (IFN+ and IL-2+) and CD8+ IFN+ cells provides a protective effect (a hazard risk of 0.28 (confidence interval 0.18 - 0.43) and 0.45 (CI 0.27 - 0.75), respectively) and the presence of corticotherapy increases the risk of reactivation (HR 2.47 (CI 1.82-3.36)). Thus, a patient without corticotherapy and with both of the critical T cell subsets present has a cumulative 19.6 times lower risk of developing CMV reactivation than a patient on corticotherapy and without CD8+ dual positive (IFN+ and IL-2+) or CD8+ IFN+ cells. ConclusionsWe have established parameters of CMV specific functional response ex vivo that can be used in assisting clinical management of patients with CMV reactivation. (c) 2015 International Clinical Cytometry Society

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

<a href="/cs/project/GA13-22777S" target="_blank" >GA13-22777S: Adoptivní transfer CMV-specifických CD8+ T-lymfocytů k léčbě CMV infekce po transplantaci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytometry Part B - Clinical Cytometry

ISSN

1552-4949

e-ISSN

—

Svazek periodika

92

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

380-389

Kód UT WoS článku

000409938500008

EID výsledku v databázi Scopus

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