Human adipose tissue accumulation is associated with pro-inflammatory changes in subcutaneous rather than visceral adipose tissue

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10373833" target="_blank" >RIV/00216208:11130/17:10373833 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/17:00075952

Výsledek na webu

<a href="https://doi.org/10.1038/nutd.2017.15" target="_blank" >https://doi.org/10.1038/nutd.2017.15</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/nutd.2017.15" target="_blank" >10.1038/nutd.2017.15</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Human adipose tissue accumulation is associated with pro-inflammatory changes in subcutaneous rather than visceral adipose tissue

Popis výsledku v původním jazyce

The importance of the involvement of adipose tissue macrophage subpopulations in obesity-related disorders is well known from different animal models, but human data are scarcer. Subcutaneous (n = 44) and visceral (n = 52) adipose tissues of healthy living kidney donors were obtained during living donor nephrectomy. Stromal vascular fractions were isolated and analysed by flow cytometry using CD14, CD16, CD36 and CD163 antibodies. Total macrophage numbers in subcutaneous adipose tissue increased (P = 0.02) with body mass index (BMI), with a similar increase seen in the proportion of phagocytic CD14+CD16+CD36(high) macrophages (P &lt; 0.01). On the other hand, there was an inverse correlation between anti-inflammatory CD14+CD16 - CD163+ macrophages (P &lt; 0.05) and BMI. These correlations disappeared after excluding obese subjects (BMI &gt;= 30 kg m(-2)) from the analysis. Interestingly, none of these subpopulations were significantly related to BMI in visceral adipose tissue. Obesity per se is associated with distinct, highly phagocytic macrophage accumulation in human subcutaneous adipose tissue.

Název v anglickém jazyce

Human adipose tissue accumulation is associated with pro-inflammatory changes in subcutaneous rather than visceral adipose tissue

Popis výsledku anglicky

The importance of the involvement of adipose tissue macrophage subpopulations in obesity-related disorders is well known from different animal models, but human data are scarcer. Subcutaneous (n = 44) and visceral (n = 52) adipose tissues of healthy living kidney donors were obtained during living donor nephrectomy. Stromal vascular fractions were isolated and analysed by flow cytometry using CD14, CD16, CD36 and CD163 antibodies. Total macrophage numbers in subcutaneous adipose tissue increased (P = 0.02) with body mass index (BMI), with a similar increase seen in the proportion of phagocytic CD14+CD16+CD36(high) macrophages (P &lt; 0.01). On the other hand, there was an inverse correlation between anti-inflammatory CD14+CD16 - CD163+ macrophages (P &lt; 0.05) and BMI. These correlations disappeared after excluding obese subjects (BMI &gt;= 30 kg m(-2)) from the analysis. Interestingly, none of these subpopulations were significantly related to BMI in visceral adipose tissue. Obesity per se is associated with distinct, highly phagocytic macrophage accumulation in human subcutaneous adipose tissue.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nutrition &amp; Diabetes [online]

ISSN

2044-4052

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

—

Kód UT WoS článku

000401107800007

EID výsledku v databázi Scopus

2-s2.0-85017365010