Sarcosine Degradation Pathway is Involved in the Epigenetics of Prostate Cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10389377" target="_blank" >RIV/00216208:11130/17:10389377 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43210/17:43912636 RIV/00216208:11310/17:10389377 RIV/00216305:26620/17:PU128385 RIV/00064203:_____/17:10389377
Výsledek na webu
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Sarcosine Degradation Pathway is Involved in the Epigenetics of Prostate Cells
Popis výsledku v původním jazyce
It has been shown that sarcosine suplementation stimulates the proliferation of prostate cells and also their invassiveness. In present study we show that enzymes conected with sarcosine conversion to glycine (sarcosine dehydrogenase, pipecolic acid oxidase) are stimulated due to sarcosine treatment. Further, sarcosine treatment increases S-adenosylmethioneine-to-S-adenosylhomocysteine ratio, which indicates a release and utilization of free methyl groups from sarcosine degradation pathway. We identified the highest induction of global methylation in non-malignant PNT1A cells, but global methylation profiles were altered also in malignant (22Rv1) and metastatic (LNCaP) cells. The influence on methylation changes was further verified using hypomethylating agent 5-azacytidine (5-aza). Co-treatment of prostate cells with 5-aza and sarcosine resulted in decrease in cells invassiveness when compared to treatment with sarcosine alone. This correlates with sarcosine-related hypermethylation of genes involved in cells growth and cell cycle.
Název v anglickém jazyce
Sarcosine Degradation Pathway is Involved in the Epigenetics of Prostate Cells
Popis výsledku anglicky
It has been shown that sarcosine suplementation stimulates the proliferation of prostate cells and also their invassiveness. In present study we show that enzymes conected with sarcosine conversion to glycine (sarcosine dehydrogenase, pipecolic acid oxidase) are stimulated due to sarcosine treatment. Further, sarcosine treatment increases S-adenosylmethioneine-to-S-adenosylhomocysteine ratio, which indicates a release and utilization of free methyl groups from sarcosine degradation pathway. We identified the highest induction of global methylation in non-malignant PNT1A cells, but global methylation profiles were altered also in malignant (22Rv1) and metastatic (LNCaP) cells. The influence on methylation changes was further verified using hypomethylating agent 5-azacytidine (5-aza). Co-treatment of prostate cells with 5-aza and sarcosine resulted in decrease in cells invassiveness when compared to treatment with sarcosine alone. This correlates with sarcosine-related hypermethylation of genes involved in cells growth and cell cycle.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
30204 - Oncology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA16-18917S" target="_blank" >GA16-18917S: Studium metabolismu sarkosinu a jeho participace na vývoji nádorů prostaty</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Proceedings of 24Th International PhD Students Conference (Mendelnet 2017)
ISBN
978-80-7509-529-9
ISSN
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e-ISSN
neuvedeno
Počet stran výsledku
5
Strana od-do
927-931
Název nakladatele
MENDEL UNIV BRNO, FAC AGRONOMY
Místo vydání
BRNO
Místo konání akce
Mendel Univ Brno, Fac AgriSciences, Brno
Datum konání akce
8. 11. 2017
Typ akce podle státní příslušnosti
CST - Celostátní akce
Kód UT WoS článku
000440194500166