Determination of vitamins K-1, MK-4, and MK-7 in human serum of postmenopausal women by HPLC with fluorescence detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10376155" target="_blank" >RIV/00216208:11130/18:10376155 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/18:10376155

Výsledek na webu

<a href="https://doi.org/10.1002/jcla.22381" target="_blank" >https://doi.org/10.1002/jcla.22381</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jcla.22381" target="_blank" >10.1002/jcla.22381</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of vitamins K-1, MK-4, and MK-7 in human serum of postmenopausal women by HPLC with fluorescence detection

Popis výsledku v původním jazyce

BackgroundNew high-performance liquid chromatography (HPLC) method was developed for the determination of vitamin K-1 and two forms of vitamin K-2 (MK-4 and MK-7) in human serum, and the levels of vitamin Kwere determined in 350 samples of postmenopausal women. MethodsVitamin K was determined by HPLC with fluorescence detection after postcolumn zinc reduction. The detection was performed at 246nm (excitation) and 430nm (emission). The internal standard and 2mL of ethanol were added to 500L of serum. The mixture was extracted with 4mL of hexane, and solid phase extraction was then used. ResultsThe HLPC method was fully validated. The intra- and interday accuracy and precision were evaluated on two QC samples by multiple analysis, and CV were less than 10%. The limit of quantification for MK-4 was found at 0.04 ng/mL, for K-1 0.03ng/mL, and for MK-7 0.03ng/mL. The mean recoveries of the corresponding compounds were 98%-110%. Serum levels of MK-4, K-1, and MK-7 in postmenopausal women with osteoporosis were 0.8900.291ng/mL, 0.433 +/- 0.394ng/mL, and 1.002 +/- 1.020ng/mL, respectively (mean +/- SD). Serum levels of MK-4, K-1, and MK-7 in postmenopausal women without osteoporosis were 0.825 +/- 0.266ng/mL, 0.493 +/- 0.399ng/mL, and 1.186 +/- 1.076ng/mL, respectively (mean +/- SD). ConclusionNew HPLC method for the determination of vitamins K-1, MK-4, and MK-7 in serum was evaluated and validated. This method is highly specific and sensitive with the low limit of quantification.

Název v anglickém jazyce

Determination of vitamins K-1, MK-4, and MK-7 in human serum of postmenopausal women by HPLC with fluorescence detection

Popis výsledku anglicky

BackgroundNew high-performance liquid chromatography (HPLC) method was developed for the determination of vitamin K-1 and two forms of vitamin K-2 (MK-4 and MK-7) in human serum, and the levels of vitamin Kwere determined in 350 samples of postmenopausal women. MethodsVitamin K was determined by HPLC with fluorescence detection after postcolumn zinc reduction. The detection was performed at 246nm (excitation) and 430nm (emission). The internal standard and 2mL of ethanol were added to 500L of serum. The mixture was extracted with 4mL of hexane, and solid phase extraction was then used. ResultsThe HLPC method was fully validated. The intra- and interday accuracy and precision were evaluated on two QC samples by multiple analysis, and CV were less than 10%. The limit of quantification for MK-4 was found at 0.04 ng/mL, for K-1 0.03ng/mL, and for MK-7 0.03ng/mL. The mean recoveries of the corresponding compounds were 98%-110%. Serum levels of MK-4, K-1, and MK-7 in postmenopausal women with osteoporosis were 0.8900.291ng/mL, 0.433 +/- 0.394ng/mL, and 1.002 +/- 1.020ng/mL, respectively (mean +/- SD). Serum levels of MK-4, K-1, and MK-7 in postmenopausal women without osteoporosis were 0.825 +/- 0.266ng/mL, 0.493 +/- 0.399ng/mL, and 1.186 +/- 1.076ng/mL, respectively (mean +/- SD). ConclusionNew HPLC method for the determination of vitamins K-1, MK-4, and MK-7 in serum was evaluated and validated. This method is highly specific and sensitive with the low limit of quantification.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Clinical Laboratory Analysis

ISSN

0887-8013

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

000435263900017

EID výsledku v databázi Scopus

2-s2.0-85048559977