Anti-inflammatory compound curcumin and mesenchymal stem cells in the treatment of spinal cord injury in rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10387175" target="_blank" >RIV/00216208:11130/18:10387175 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/18:00507350

Výsledek na webu

<a href="https://doi.org/10.21307/ane-2018-035" target="_blank" >https://doi.org/10.21307/ane-2018-035</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21307/ane-2018-035" target="_blank" >10.21307/ane-2018-035</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Anti-inflammatory compound curcumin and mesenchymal stem cells in the treatment of spinal cord injury in rats

Popis výsledku v původním jazyce

Spinal cord injury leads to a robust inflammatory response that Is an unfavorable environment for stem cell implantation. In this study, we evaluated the effect of combined therapy of curcumin and mesenchymal stem cells (MSC) on behavioral recovery and tissue sparing, glial scar formation, axonal sprouting and inflammatory responses in a rat experimental model of spinal cord injury (SCI). Balloon-induced compression lesion was performed at thoracic (Th8-9) spinal level. Out of the four groups studied, two groups received curcumin on the surface of the spinal cord immediately after SCI and then once a week for 3 weeks together with an intraperitoneal daily curcumin injection for 28 days. The other two groups received saline. Seven days after SCI, human MSC were intrathecally implanted in one curcumin and one saline group. Both curcumin and curcumin combined with MSC treatment improved locomotor ability in comparison to the saline treated animals. The combined treatment group showed additional improvement in advanced locomotor performance. The combined therapy facilitated axonal sprouting, and modulated expression of pro-regenerative factors and inflammatory responses, when compared to saline and single treatments. These results demonstrate that preconditioning with curcumin, prior to the MSC implantation could have a synergic effect in the treatment of experimental SCI.

Název v anglickém jazyce

Anti-inflammatory compound curcumin and mesenchymal stem cells in the treatment of spinal cord injury in rats

Popis výsledku anglicky

Spinal cord injury leads to a robust inflammatory response that Is an unfavorable environment for stem cell implantation. In this study, we evaluated the effect of combined therapy of curcumin and mesenchymal stem cells (MSC) on behavioral recovery and tissue sparing, glial scar formation, axonal sprouting and inflammatory responses in a rat experimental model of spinal cord injury (SCI). Balloon-induced compression lesion was performed at thoracic (Th8-9) spinal level. Out of the four groups studied, two groups received curcumin on the surface of the spinal cord immediately after SCI and then once a week for 3 weeks together with an intraperitoneal daily curcumin injection for 28 days. The other two groups received saline. Seven days after SCI, human MSC were intrathecally implanted in one curcumin and one saline group. Both curcumin and curcumin combined with MSC treatment improved locomotor ability in comparison to the saline treated animals. The combined treatment group showed additional improvement in advanced locomotor performance. The combined therapy facilitated axonal sprouting, and modulated expression of pro-regenerative factors and inflammatory responses, when compared to saline and single treatments. These results demonstrate that preconditioning with curcumin, prior to the MSC implantation could have a synergic effect in the treatment of experimental SCI.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Neurobiologiae Experimentalis

ISSN

0065-1400

e-ISSN

—

Svazek periodika

78

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

17

Strana od-do

358-374

Kód UT WoS článku

000454991600009

EID výsledku v databázi Scopus

2-s2.0-85059796951