Methods to assess DC-dependent priming of T cell responses by dying cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F20%3A10410922" target="_blank" >RIV/00216208:11130/20:10410922 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/20:10410922

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=J93ELXLp72" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=J93ELXLp72</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/bs.mie.2019.05.045" target="_blank" >10.1016/bs.mie.2019.05.045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Methods to assess DC-dependent priming of T cell responses by dying cells

Popis výsledku v původním jazyce

Dendritic cells have been widely investigated in cancer immunotherapy clinical trials for the last two decades mainly due to their robust ability to elicit an adaptive anticancer immune response of the cellular and humoral types. Immature DCs can be easily loaded with desired antigens. However, to become efficient antigen-presenting cells, DCs must first undergo a process of maturation. Protocols for the generation of DCs for use in cancer immunotherapy, including the generation of a large number of immature DCs for antigen pulsing and the selection of a well-defined immunostimulatory agent to achieve complete and reproducible maturation, which is a crucial step for further stimulation of T cell activation, must carefully consider the characteristics of DC physiology. In this report, we provided a detailed protocol for DC generation, pulsation and activation with the subsequent induction of T cell-specific immune responses. (C) 2020 Elsevier Inc.

Název v anglickém jazyce

Methods to assess DC-dependent priming of T cell responses by dying cells

Popis výsledku anglicky

Dendritic cells have been widely investigated in cancer immunotherapy clinical trials for the last two decades mainly due to their robust ability to elicit an adaptive anticancer immune response of the cellular and humoral types. Immature DCs can be easily loaded with desired antigens. However, to become efficient antigen-presenting cells, DCs must first undergo a process of maturation. Protocols for the generation of DCs for use in cancer immunotherapy, including the generation of a large number of immature DCs for antigen pulsing and the selection of a well-defined immunostimulatory agent to achieve complete and reproducible maturation, which is a crucial step for further stimulation of T cell activation, must carefully consider the characteristics of DC physiology. In this report, we provided a detailed protocol for DC generation, pulsation and activation with the subsequent induction of T cell-specific immune responses. (C) 2020 Elsevier Inc.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Methods in Enzymology

ISSN

0076-6879

e-ISSN

—

Svazek periodika

632

Číslo periodika v rámci svazku

Cellular Methods Pt B

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

55-65

Kód UT WoS článku

000556278200006

EID výsledku v databázi Scopus

2-s2.0-85068227265