The association of pili with the emergence and replacement of the major antibiotic resistant pneumococcal clones

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F20%3A10417380" target="_blank" >RIV/00216208:11130/20:10417380 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/20:10417380 RIV/00064211:_____/20:W0000009 RIV/00179906:_____/20:10417380 RIV/75010330:_____/20:00013307

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=KpqXGReNYB" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=KpqXGReNYB</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jmii.2019.03.007" target="_blank" >10.1016/j.jmii.2019.03.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The association of pili with the emergence and replacement of the major antibiotic resistant pneumococcal clones

Popis výsledku v původním jazyce

Background: The aim of this study was to evaluate the presence of pilus islet 1 (PI-1) and to determine its Glade type in pneumococcal isolates with reduced susceptibility to penicillin (penicillin non-susceptible pneumococci - PNSP) and/or resistant to macrolides isolated prior to and after the introduction of pneumococcal conjugate vaccines (PCVs) in the Czech Republic. Methods: Clinical isolates of serotypes 9V (n = 68) and 19A (n = 89) were examined. Isolates were characterised by multilocus sequence typing (MLST). The presence of PI-1 was determined by screening for the sortase B, C, and D genes located within PI-1. In the presence of PI-1 pilus, Glade types were classified by PCR. Results: In the pre-PCV period (2000-2007), the prevalence of PNSP was 3.9% and 2.7% of isolates were resistant to erythromycin. During 2012-2015 (post-PCV period), the rates of PNSP remained stable (3.6%), but resistance to erythromycin increased to 8.3%. While in 2000 -2007, resistance to antibiotics was associated mainly with serotype 9V, in 2012-2015, it was replaced by serotype 19A. PI-1 positive isolates were seen in both serotypes. All isolates (68) of serotype 9V belonged to the Spain( 9V)-3 (CC156) clone and carried PI-1 of Glade type I while 96.5% (56/58) of isolates of 19A serotype belonged to the Netherlands (15B)-37 (CC199) clone and carried PI-1 of Glade type II. Conclusions: Both major antibiotic resistant clones carried PI-1, although they differ in the Glade type. Thus the role of PI-1 should be evaluated in further studies and potentially considered in the spread of antibiotic resistant clones. Copyright (C) 2019, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.

Název v anglickém jazyce

The association of pili with the emergence and replacement of the major antibiotic resistant pneumococcal clones

Popis výsledku anglicky

Background: The aim of this study was to evaluate the presence of pilus islet 1 (PI-1) and to determine its Glade type in pneumococcal isolates with reduced susceptibility to penicillin (penicillin non-susceptible pneumococci - PNSP) and/or resistant to macrolides isolated prior to and after the introduction of pneumococcal conjugate vaccines (PCVs) in the Czech Republic. Methods: Clinical isolates of serotypes 9V (n = 68) and 19A (n = 89) were examined. Isolates were characterised by multilocus sequence typing (MLST). The presence of PI-1 was determined by screening for the sortase B, C, and D genes located within PI-1. In the presence of PI-1 pilus, Glade types were classified by PCR. Results: In the pre-PCV period (2000-2007), the prevalence of PNSP was 3.9% and 2.7% of isolates were resistant to erythromycin. During 2012-2015 (post-PCV period), the rates of PNSP remained stable (3.6%), but resistance to erythromycin increased to 8.3%. While in 2000 -2007, resistance to antibiotics was associated mainly with serotype 9V, in 2012-2015, it was replaced by serotype 19A. PI-1 positive isolates were seen in both serotypes. All isolates (68) of serotype 9V belonged to the Spain( 9V)-3 (CC156) clone and carried PI-1 of Glade type I while 96.5% (56/58) of isolates of 19A serotype belonged to the Netherlands (15B)-37 (CC199) clone and carried PI-1 of Glade type II. Conclusions: Both major antibiotic resistant clones carried PI-1, although they differ in the Glade type. Thus the role of PI-1 should be evaluated in further studies and potentially considered in the spread of antibiotic resistant clones. Copyright (C) 2019, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV16-27109A" target="_blank" >NV16-27109A: Vývoj multirezistentního Streptococcus pneumoniae sérotypu 19A v éře vakcinace proti pneumokokům</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiology, Immunology and Infection

ISSN

1684-1182

e-ISSN

—

Svazek periodika

53

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

TW - Čínská republika (Tchaj-wan)

Počet stran výsledku

6

Strana od-do

690-695

Kód UT WoS článku

000576693200005

EID výsledku v databázi Scopus

2-s2.0-85064449040