Identification of bacterial profiles and their interactions with selected quality, oxidative, and immunological parameters of turkey semen

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F21%3A10428668" target="_blank" >RIV/00216208:11130/21:10428668 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=A_nwbBizRk" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=A_nwbBizRk</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani11061771" target="_blank" >10.3390/ani11061771</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of bacterial profiles and their interactions with selected quality, oxidative, and immunological parameters of turkey semen

Popis výsledku v původním jazyce

This study focused on the identification of naturally occurring bacteria in the reproductive fluid and impact on the quality of ejaculates obtained from the turkey breed British United Turkeys (BUT) Big 6 (n = 60). We determined possible relationships between the bacterial load and advanced sperm quality parameters that are important for effective artificial insemination and high fertility, as well as the concentration of selected antimicrobial proteins and pro-inflammatory markers of turkey semen. Sperm motility was assessed with computer-assisted sperm analysis (CASA), while the membrane and acrosome integrity were examined with smearing and staining methods. Reactive oxygen species (ROS) generation was quantified via luminometry, sperm DNA fragmentation was evaluated using the TUNEL assay, and the JC-1 assay was applied to evaluate the mitochondrial membrane potential. Cell lysates were prepared to investigate the extent of lipid and protein oxidation. Furthermore, levels of interleukins 1 and 6 (IL-1, IL-6), C-reactive protein, cathelicidin, and β-defensin were quantified in the seminal plasma using the ELISA method. The most dominant species identified by the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry was Escherichia coli, Proteus mirabilis, Staphylococcus lentus, and Citrobacter braakii. The bacterial load had a negative effect on the sperm motility (p &lt; 0.001), as well as membrane (p &lt; 0.05) and acrosome integrity (p &lt; 0.01). A strong positive relationship between the bacterial load and DNA fragmentation (p &lt; 0.001) was detected as well. Positive associations were recorded between the increasing presence of bacteria, ROS overgeneration (p &lt; 0.001), and a subsequent oxidative damage to the proteins (p &lt; 0.001) and lipids (p &lt; 0.01). It was revealed that the antimicrobial peptides β-defensin (p &lt; 0.001) and cathelicidin (p &lt; 0.001) had a positive relationship with the motility. In contrast, pro-inflammatory markers, such as IL-1 (p &lt; 0.001) and IL-6 (p &lt; 0.001), had a negative impact on the motion behavior of turkey spermatozoa. Our results suggest that the semen quality may be notably affected by the bacterial quantity as well as quality. It seems that bacteriospermia is associated with inflammatory processes, oxidative stress, sperm structural deterioration, and a subsequent risk for a failed artificial insemination in turkey breeding.

Název v anglickém jazyce

Identification of bacterial profiles and their interactions with selected quality, oxidative, and immunological parameters of turkey semen

Popis výsledku anglicky

This study focused on the identification of naturally occurring bacteria in the reproductive fluid and impact on the quality of ejaculates obtained from the turkey breed British United Turkeys (BUT) Big 6 (n = 60). We determined possible relationships between the bacterial load and advanced sperm quality parameters that are important for effective artificial insemination and high fertility, as well as the concentration of selected antimicrobial proteins and pro-inflammatory markers of turkey semen. Sperm motility was assessed with computer-assisted sperm analysis (CASA), while the membrane and acrosome integrity were examined with smearing and staining methods. Reactive oxygen species (ROS) generation was quantified via luminometry, sperm DNA fragmentation was evaluated using the TUNEL assay, and the JC-1 assay was applied to evaluate the mitochondrial membrane potential. Cell lysates were prepared to investigate the extent of lipid and protein oxidation. Furthermore, levels of interleukins 1 and 6 (IL-1, IL-6), C-reactive protein, cathelicidin, and β-defensin were quantified in the seminal plasma using the ELISA method. The most dominant species identified by the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry was Escherichia coli, Proteus mirabilis, Staphylococcus lentus, and Citrobacter braakii. The bacterial load had a negative effect on the sperm motility (p &lt; 0.001), as well as membrane (p &lt; 0.05) and acrosome integrity (p &lt; 0.01). A strong positive relationship between the bacterial load and DNA fragmentation (p &lt; 0.001) was detected as well. Positive associations were recorded between the increasing presence of bacteria, ROS overgeneration (p &lt; 0.001), and a subsequent oxidative damage to the proteins (p &lt; 0.001) and lipids (p &lt; 0.01). It was revealed that the antimicrobial peptides β-defensin (p &lt; 0.001) and cathelicidin (p &lt; 0.001) had a positive relationship with the motility. In contrast, pro-inflammatory markers, such as IL-1 (p &lt; 0.001) and IL-6 (p &lt; 0.001), had a negative impact on the motion behavior of turkey spermatozoa. Our results suggest that the semen quality may be notably affected by the bacterial quantity as well as quality. It seems that bacteriospermia is associated with inflammatory processes, oxidative stress, sperm structural deterioration, and a subsequent risk for a failed artificial insemination in turkey breeding.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animals [online]

ISSN

2076-2615

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

1771

Kód UT WoS článku

000665283000001

EID výsledku v databázi Scopus

2-s2.0-85107729056