Lohmann Brown Rooster Semen: Intrinsic Bacteria and Their Impact on Sperm Progressive Motility and Seminal Biochemical Parameters-A Preliminary Study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F23%3A10477091" target="_blank" >RIV/00216208:11130/23:10477091 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GPfXCpJlV4" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GPfXCpJlV4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/stresses3020031" target="_blank" >10.3390/stresses3020031</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lohmann Brown Rooster Semen: Intrinsic Bacteria and Their Impact on Sperm Progressive Motility and Seminal Biochemical Parameters-A Preliminary Study

Popis výsledku v původním jazyce

Semen quality plays a crucial role in poultry production; however, it may be impaired by the presence of numerous bacterial species. This study researched the impact of bacterial contamination of Lohmann brown rooster semen on the biochemical parameters of seminal plasma to evaluate its potential consequences on the sperm progressive motility. Semen was collected from 27 stud roosters, and the sperm concentration and progressive motility were measured using computer-assisted semen analysis (CASA). Seminal plasma was separated, and selected biochemical parameters were measured using commercially available assays. An aliquot of each semen sample was cultured, the colonies were counted and the MALDI Biotyper was used for bacterial identification. The samples were divided into three categories based on their sperm progressive motility and the data were compared and statistically evaluated. Moreover, Pearson&apos;s correlation analysis was performed. The results showed that the lower the sperm progressive motility, the higher the level of colony-forming units. Moreover, sperm concentration was significantly higher (p &lt; 0.05) in the group with the highest bacterial occurrence and the lowest proportion of progressively motile spermatozoa. Calcium, magnesium, creatinine, uric acid, alkaline phosphatase, and total proteins significantly changed in semen samples with the lowest proportion of progressive motility. In conclusion, seminal plasma biochemistry may mirror changes occurring in semen as a result of bacterial presence in the reproductive tract of poultry.

Název v anglickém jazyce

Lohmann Brown Rooster Semen: Intrinsic Bacteria and Their Impact on Sperm Progressive Motility and Seminal Biochemical Parameters-A Preliminary Study

Popis výsledku anglicky

Semen quality plays a crucial role in poultry production; however, it may be impaired by the presence of numerous bacterial species. This study researched the impact of bacterial contamination of Lohmann brown rooster semen on the biochemical parameters of seminal plasma to evaluate its potential consequences on the sperm progressive motility. Semen was collected from 27 stud roosters, and the sperm concentration and progressive motility were measured using computer-assisted semen analysis (CASA). Seminal plasma was separated, and selected biochemical parameters were measured using commercially available assays. An aliquot of each semen sample was cultured, the colonies were counted and the MALDI Biotyper was used for bacterial identification. The samples were divided into three categories based on their sperm progressive motility and the data were compared and statistically evaluated. Moreover, Pearson&apos;s correlation analysis was performed. The results showed that the lower the sperm progressive motility, the higher the level of colony-forming units. Moreover, sperm concentration was significantly higher (p &lt; 0.05) in the group with the highest bacterial occurrence and the lowest proportion of progressively motile spermatozoa. Calcium, magnesium, creatinine, uric acid, alkaline phosphatase, and total proteins significantly changed in semen samples with the lowest proportion of progressive motility. In conclusion, seminal plasma biochemistry may mirror changes occurring in semen as a result of bacterial presence in the reproductive tract of poultry.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Stresses

ISSN

2673-7140

e-ISSN

—

Svazek periodika

3

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

424-433

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85183758783