Mesenchymal Stem Cells Attenuate Renal Microscopic Alterations in Induced Diabetic Nephropathy in Rats through Suppression of Oxidative Stress, Inflammation, Apoptosis and Upregulation of Nrf2/PPAR-γ Inflammatory Signaling Pathway

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F25%3A10496732" target="_blank" >RIV/00216208:11130/25:10496732 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=64UQhsXJ3I" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=64UQhsXJ3I</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4067/S0717-95022025000100226" target="_blank" >10.4067/S0717-95022025000100226</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mesenchymal Stem Cells Attenuate Renal Microscopic Alterations in Induced Diabetic Nephropathy in Rats through Suppression of Oxidative Stress, Inflammation, Apoptosis and Upregulation of Nrf2/PPAR-γ Inflammatory Signaling Pathway

Popis výsledku v původním jazyce

Diabetic nephropathy (DN) is a prevalent complication of diabetes, necessitating the development of effective therapies targeting the mechanisms by which type 2 diabetes mellitus (T2DM) induces renal tissue damage. In this study, DN was induced in rats using a high-fat diet for 13 weeks combined with streptozotocin to assess the effects of mesenchymal stem(MSCs) cells injection on renal tissues and function. Histological, immunohistochemistry, and biochemical analysis were employed to evaluate inflammation, oxidative stress, apoptosis, and histological architecture. The results demonstrated that MSCs improved metabolic derangement (glucose and lipid profile) and kidney function (urea and creatinine) associated with a significant reduction inflammatoryin biomarkers: high sensitivity CRP (hs-CRP), tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), as well as oxidative stress biomarkers :reactive: oxygen species (ROS) and superoxide dismutase (SOD). Additionally, MSCs administration upregulates omeperoxi proliferator-activated receptor gamma (PPAR gamma) and nuclear factor erythroid 2-related factor 2 (Nrf2) expression levels. MSCs also show improved histological architecture associated with decreased CD45 (marker of inflammation) and caspase-3 (marker of apoptosis) immunostaining in renal tissues of the treated diabetic group. In conclusion, MSCs improved diabetic-induced nephropathyh throug suppression of oxidative stress, inflammation, apoptosis and upregulation of the Nrf2/PPAR-gamma inflammatory signaling pathway.

Název v anglickém jazyce

Mesenchymal Stem Cells Attenuate Renal Microscopic Alterations in Induced Diabetic Nephropathy in Rats through Suppression of Oxidative Stress, Inflammation, Apoptosis and Upregulation of Nrf2/PPAR-γ Inflammatory Signaling Pathway

Popis výsledku anglicky

Diabetic nephropathy (DN) is a prevalent complication of diabetes, necessitating the development of effective therapies targeting the mechanisms by which type 2 diabetes mellitus (T2DM) induces renal tissue damage. In this study, DN was induced in rats using a high-fat diet for 13 weeks combined with streptozotocin to assess the effects of mesenchymal stem(MSCs) cells injection on renal tissues and function. Histological, immunohistochemistry, and biochemical analysis were employed to evaluate inflammation, oxidative stress, apoptosis, and histological architecture. The results demonstrated that MSCs improved metabolic derangement (glucose and lipid profile) and kidney function (urea and creatinine) associated with a significant reduction inflammatoryin biomarkers: high sensitivity CRP (hs-CRP), tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), as well as oxidative stress biomarkers :reactive: oxygen species (ROS) and superoxide dismutase (SOD). Additionally, MSCs administration upregulates omeperoxi proliferator-activated receptor gamma (PPAR gamma) and nuclear factor erythroid 2-related factor 2 (Nrf2) expression levels. MSCs also show improved histological architecture associated with decreased CD45 (marker of inflammation) and caspase-3 (marker of apoptosis) immunostaining in renal tissues of the treated diabetic group. In conclusion, MSCs improved diabetic-induced nephropathyh throug suppression of oxidative stress, inflammation, apoptosis and upregulation of the Nrf2/PPAR-gamma inflammatory signaling pathway.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30106 - Anatomy and morphology (plant science to be 1.6)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Morphology

ISSN

0717-9367

e-ISSN

0717-9502

Svazek periodika

43

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CL - Chilská republika

Počet stran výsledku

11

Strana od-do

226-236

Kód UT WoS článku

001450583300030

EID výsledku v databázi Scopus

2-s2.0-105001432960