Immunohistochemical detection and quantification of T cells in the small intestine of Isospora suis-infected piglets-influence of fixation technique and intestinal segment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F12%3A10118804" target="_blank" >RIV/00216208:11140/12:10118804 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/49777513:23520/12:43914832

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jemt.21071/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jemt.21071/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jemt.21071" target="_blank" >10.1002/jemt.21071</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunohistochemical detection and quantification of T cells in the small intestine of Isospora suis-infected piglets-influence of fixation technique and intestinal segment

Popis výsledku v původním jazyce

Quantification of immunohistochemical results constitutes an important tool in the analysis of cells and tissue that is not readily replaced by other techniques. For reliable quantification, it is essential to consider factors such as tissue fixation andtissue sampling. We report a study on the model of the intestine of Isospora suis-infected piglets, in which we addressed (1) whether the quantity of detectable T cells in the intestinal mucosa is the same in formalin-, HOPE(R)-, and cryo-conserved material or whether the amounts of T cells at least correlate with one another; and (2) whether single jejunal segments differ in regard to the quantity of mucosal T cells and variability of lymphocyte infiltration. Quantification of T cells in histologicalsections of different parts of the jejunum of 15-22 day old piglets infected with I. suis was performed using an anti-CD3-antibody and stereological point counting. Area fractions of T-cell profiles per intestinal mucosa profile were high

Název v anglickém jazyce

Immunohistochemical detection and quantification of T cells in the small intestine of Isospora suis-infected piglets-influence of fixation technique and intestinal segment

Popis výsledku anglicky

Quantification of immunohistochemical results constitutes an important tool in the analysis of cells and tissue that is not readily replaced by other techniques. For reliable quantification, it is essential to consider factors such as tissue fixation andtissue sampling. We report a study on the model of the intestine of Isospora suis-infected piglets, in which we addressed (1) whether the quantity of detectable T cells in the intestinal mucosa is the same in formalin-, HOPE(R)-, and cryo-conserved material or whether the amounts of T cells at least correlate with one another; and (2) whether single jejunal segments differ in regard to the quantity of mucosal T cells and variability of lymphocyte infiltration. Quantification of T cells in histologicalsections of different parts of the jejunum of 15-22 day old piglets infected with I. suis was performed using an anti-CD3-antibody and stereological point counting. Area fractions of T-cell profiles per intestinal mucosa profile were high

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

<a href="/cs/project/ME09090" target="_blank" >ME09090: Design kvantitativních studií v histologii - strategie vzorkování a detekce struktur, testování spolehlivosti a reprodukovatelnosti kvantitativních analýz</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy Research and Technique

ISSN

1059-910X

e-ISSN

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Svazek periodika

75

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

408-415

Kód UT WoS článku

000301923000002

EID výsledku v databázi Scopus

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