Low Frequency of HIF-1 alpha Overexpression in Germ Cell Tumors of the Testis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F13%3A10134073" target="_blank" >RIV/00216208:11140/13:10134073 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00669806:_____/13:10134073

Výsledek na webu

<a href="http://dx.doi.org/10.1097/PAI.0b013e31825e00b7" target="_blank" >http://dx.doi.org/10.1097/PAI.0b013e31825e00b7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1097/PAI.0b013e31825e00b7" target="_blank" >10.1097/PAI.0b013e31825e00b7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Low Frequency of HIF-1 alpha Overexpression in Germ Cell Tumors of the Testis

Popis výsledku v původním jazyce

Cellular hypoxia is a hallmark of cancer. Hypoxia-inducible factor-1 alpha (HIF-1 alpha) and von Hippel-Lindau protein (pVHL) are the key mediators of cellular response to hypoxia. Little is known about their role in germ cell tumors of the testis. We therefore examined their status in a cohort of germ cell tumors of the testis. Thirty-six primary germ cell tumors of the testis (11 seminomas, 24 mixed germ cell tumors, and 1 case of pure intratubular germ cell neoplasia) were included in the study. HIF-1 alpha and pVHL expression were studied using immunohistochemical (IHC) methods in the tumor and adjacent benign tissue. Selected cases with a low pVHL expression were further tested for genetic alterations using polymerase chain reaction. HIF-1 alpha protein expression was not detectable in adjacent atrophic seminiferous tubules. In contrast, HIF-1 alpha was expressed in one third of the malignancies, but in a low percentage of cells (mean, 3%; range, 0% to 20%). No difference in HIF-1

Název v anglickém jazyce

Low Frequency of HIF-1 alpha Overexpression in Germ Cell Tumors of the Testis

Popis výsledku anglicky

Cellular hypoxia is a hallmark of cancer. Hypoxia-inducible factor-1 alpha (HIF-1 alpha) and von Hippel-Lindau protein (pVHL) are the key mediators of cellular response to hypoxia. Little is known about their role in germ cell tumors of the testis. We therefore examined their status in a cohort of germ cell tumors of the testis. Thirty-six primary germ cell tumors of the testis (11 seminomas, 24 mixed germ cell tumors, and 1 case of pure intratubular germ cell neoplasia) were included in the study. HIF-1 alpha and pVHL expression were studied using immunohistochemical (IHC) methods in the tumor and adjacent benign tissue. Selected cases with a low pVHL expression were further tested for genetic alterations using polymerase chain reaction. HIF-1 alpha protein expression was not detectable in adjacent atrophic seminiferous tubules. In contrast, HIF-1 alpha was expressed in one third of the malignancies, but in a low percentage of cells (mean, 3%; range, 0% to 20%). No difference in HIF-1

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

<a href="/cs/project/NT12010" target="_blank" >NT12010: Stanovení genetických změn v průběhu angiogeneze u různých typů nádorů ledvin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Immunohistochemistry

ISSN

1062-3345

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

165-169

Kód UT WoS článku

000315464500010

EID výsledku v databázi Scopus

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