The influence of chronic administration of thioacetamide on levels of ALT, AST and GLDH in serum and markers of oxidative stress in the liver and kidneys of female Wistar rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F13%3A10139295" target="_blank" >RIV/00216208:11140/13:10139295 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The influence of chronic administration of thioacetamide on levels of ALT, AST and GLDH in serum and markers of oxidative stress in the liver and kidneys of female Wistar rats

Popis výsledku v původním jazyce

Background. Thioacetamide (TAA), a well known inducer of hepatic fibrosis, has been often used in experimental acute and chronic hepatotoxicity models. Reactive oxygen species play an important role in TAA-induced liver damage. Aims. To study the time course of activities of ALT, AST and GLDH in serum and markers of oxidative stress in the liver and kidneys of rats after chronic TAA administration. Methods. Female Wistar rats were treated with TAA (intraperitoneally, 200 mg/kg body weight) for 12 weeks,the controls received saline. Animals were sacrificed 4, 12 or 16 weeks after the treatment discontinuation. Lipid peroxidation (LP), reduced glutathione (GSH) and activities of glutathione peroxidase (GPx), glutathione reductase (GR) and katalase (CAT)were estimated in the liver and kidneys. ALT, AST and GLDH were measured in serum. Results. Significant increases in serum ALT and GLDH persisted for 16 weeks ater the treatment, while serum AST was increased only in animals sacrificed 4

Název v anglickém jazyce

The influence of chronic administration of thioacetamide on levels of ALT, AST and GLDH in serum and markers of oxidative stress in the liver and kidneys of female Wistar rats

Popis výsledku anglicky

Background. Thioacetamide (TAA), a well known inducer of hepatic fibrosis, has been often used in experimental acute and chronic hepatotoxicity models. Reactive oxygen species play an important role in TAA-induced liver damage. Aims. To study the time course of activities of ALT, AST and GLDH in serum and markers of oxidative stress in the liver and kidneys of rats after chronic TAA administration. Methods. Female Wistar rats were treated with TAA (intraperitoneally, 200 mg/kg body weight) for 12 weeks,the controls received saline. Animals were sacrificed 4, 12 or 16 weeks after the treatment discontinuation. Lipid peroxidation (LP), reduced glutathione (GSH) and activities of glutathione peroxidase (GPx), glutathione reductase (GR) and katalase (CAT)were estimated in the liver and kidneys. ALT, AST and GLDH were measured in serum. Results. Significant increases in serum ALT and GLDH persisted for 16 weeks ater the treatment, while serum AST was increased only in animals sacrificed 4

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F03.0076" target="_blank" >ED2.1.00/03.0076: Biomedicínské centrum Lékařské fakulty v Plzni</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Svazek periodika

157

Číslo periodika v rámci svazku

Supplement 1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

"S65"-"S69"

Kód UT WoS článku

000324728600005

EID výsledku v databázi Scopus

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