Expanded cryopreserved mesenchymal stromal cells as an optimal source for graft-versus-host disease treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F14%3A10218654" target="_blank" >RIV/00216208:11140/14:10218654 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/14:00435673 RIV/00669806:_____/14:10218654

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.biologicals.2014.01.003" target="_blank" >http://dx.doi.org/10.1016/j.biologicals.2014.01.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biologicals.2014.01.003" target="_blank" >10.1016/j.biologicals.2014.01.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expanded cryopreserved mesenchymal stromal cells as an optimal source for graft-versus-host disease treatment

Popis výsledku v původním jazyce

Mesenchymal stromal cells (MSC) are fibroblast-like cells present in different types of tissues. Their immunomodulatory potential represents a promising method for post-transplant immunotherapy in the treatment of GVHD (graft-versus-host disease) with suboptimal response to standard immunosuppression. In this study we tested influence of 1e8 month-long cryopreservation on ability of MSC to suppress activation of non-specifically stimulated lymphocytes. We did not observe any changes in proliferation capacity of MSC after thawing. Lymphocytes metabolic activity was inhibited by 30% and number of dividing cells was three times smaller in the presence of MSC. Two activation markers were studied (CD25 and CD69) to confirm preservation of functional cell integrity. Expression of CD25 antigen on CD3ţCD4ţ and CD3ţCD4 cells was decreased in all cocultivated samples. Level of CD69 expression on CD3ţCD4ţ cells was lower in samples with added MSC (10e15% on day ţ2) but without reaching statistica

Název v anglickém jazyce

Expanded cryopreserved mesenchymal stromal cells as an optimal source for graft-versus-host disease treatment

Popis výsledku anglicky

Mesenchymal stromal cells (MSC) are fibroblast-like cells present in different types of tissues. Their immunomodulatory potential represents a promising method for post-transplant immunotherapy in the treatment of GVHD (graft-versus-host disease) with suboptimal response to standard immunosuppression. In this study we tested influence of 1e8 month-long cryopreservation on ability of MSC to suppress activation of non-specifically stimulated lymphocytes. We did not observe any changes in proliferation capacity of MSC after thawing. Lymphocytes metabolic activity was inhibited by 30% and number of dividing cells was three times smaller in the presence of MSC. Two activation markers were studied (CD25 and CD69) to confirm preservation of functional cell integrity. Expression of CD25 antigen on CD3ţCD4ţ and CD3ţCD4 cells was decreased in all cocultivated samples. Level of CD69 expression on CD3ţCD4ţ cells was lower in samples with added MSC (10e15% on day ţ2) but without reaching statistica

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F03.0076" target="_blank" >ED2.1.00/03.0076: Biomedicínské centrum Lékařské fakulty v Plzni</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biologicals

ISSN

1045-1056

e-ISSN

—

Svazek periodika

42

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

139-144

Kód UT WoS článku

000337775200002

EID výsledku v databázi Scopus

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