Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F14%3A10284523" target="_blank" >RIV/00216208:11140/14:10284523 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/49777513:23520/14:43923974

Výsledek na webu

<a href="http://www.engmech.cz/2014/im/doc/EM2014_proceedings.pdf" target="_blank" >http://www.engmech.cz/2014/im/doc/EM2014_proceedings.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections

Popis výsledku v původním jazyce

Aims of our study were as follows: 1) to perform a 3D reconstruction of parts of the adjacent classical hepatic lobules, 2) to compare the histomorphometric techniques available for estimating the volume of the lobules using 3D reconstruction and interactive stereological techniques, 3) to assess the volume fraction of liver parenchyma, connective tissue, and blood vessels, 4) to quantify the shrinkage in tissue blocks porcine liver. The morphometric parameters were assessed using serial histological sections and stereological methods. Volume of the sampled portions of the lobules ranged between 0.282-0.498 mm3. The volume fraction of parenchyma in liver was 0.867, the volume fraction of connective tissue was 0.077and the fraction of blood vessels was 0.026. The mean volume shrinkage of porcine liver was 55.23%. This value can be used for correcting the data based on paraffin-processed histological sections. For further quantitative studies, we suggest the nucleator technique as a fast, robust and reliable method for estimating the volume of the lobules. For biomechanical modelling, the absolute volumes resulting from histological studies have to be corrected due to the tissue shrinkage affecting the paraffin-processed tissue samples.

Název v anglickém jazyce

Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections

Popis výsledku anglicky

Aims of our study were as follows: 1) to perform a 3D reconstruction of parts of the adjacent classical hepatic lobules, 2) to compare the histomorphometric techniques available for estimating the volume of the lobules using 3D reconstruction and interactive stereological techniques, 3) to assess the volume fraction of liver parenchyma, connective tissue, and blood vessels, 4) to quantify the shrinkage in tissue blocks porcine liver. The morphometric parameters were assessed using serial histological sections and stereological methods. Volume of the sampled portions of the lobules ranged between 0.282-0.498 mm3. The volume fraction of parenchyma in liver was 0.867, the volume fraction of connective tissue was 0.077and the fraction of blood vessels was 0.026. The mean volume shrinkage of porcine liver was 55.23%. This value can be used for correcting the data based on paraffin-processed histological sections. For further quantitative studies, we suggest the nucleator technique as a fast, robust and reliable method for estimating the volume of the lobules. For biomechanical modelling, the absolute volumes resulting from histological studies have to be corrected due to the tissue shrinkage affecting the paraffin-processed tissue samples.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

30106 - Anatomy and morphology (plant science to be 1.6)

Projekt

<a href="/cs/project/ED2.1.00%2F03.0076" target="_blank" >ED2.1.00/03.0076: Biomedicínské centrum Lékařské fakulty v Plzni</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Enginnering Mechanics 2014

ISBN

978-80-214-4871-1

ISSN

1805-8248

e-ISSN

neuvedeno

Počet stran výsledku

4

Strana od-do

340-343

Název nakladatele

VUTB - Vysoké učení technické v Brně

Místo vydání

Brno

Místo konání akce

Svratka, Česká republika

Datum konání akce

12. 5. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000364573900078