Optimized cutting laser trajectory for laser capture microdissection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F19%3A10394240" target="_blank" >RIV/00216208:11140/19:10394240 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10394240

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=VsA3k-lJSw" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=VsA3k-lJSw</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11756-019-00234-x" target="_blank" >10.2478/s11756-019-00234-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimized cutting laser trajectory for laser capture microdissection

Popis výsledku v původním jazyce

Laser capture microdissection (LCM) is an excellent tool using a laser beam for specific selection and harvesting of cells or cell populations from heterogeneous tissue sections prepared on special slides. The aim of our study was to apply mathematical methods for optimizing the planning of laser trajectory in LCM combining positive and negative selection criteria with adjustable size, weight, importance, and security borders. We developed and tested software tool named CutPlanner to be used in a transparent overlay superimposed to the live camera image independently from the manufacturer of the LCM device. Once optimized, quantified and approved by the user, the resulting outline of the region of interest is directly copied to the laser beam cutting trajectory. The software is made publicly available for non-commercial use to the scientific community and provides a versatile tool for effectively minimizing the length of the laser trajectory to obtain the selected cells without destroying surrounding cells and tissue structures. Saving all the settings allows for performing repeating tasks under similar conditions, especially in uniform and routinely performed LCM applications.

Název v anglickém jazyce

Optimized cutting laser trajectory for laser capture microdissection

Popis výsledku anglicky

Laser capture microdissection (LCM) is an excellent tool using a laser beam for specific selection and harvesting of cells or cell populations from heterogeneous tissue sections prepared on special slides. The aim of our study was to apply mathematical methods for optimizing the planning of laser trajectory in LCM combining positive and negative selection criteria with adjustable size, weight, importance, and security borders. We developed and tested software tool named CutPlanner to be used in a transparent overlay superimposed to the live camera image independently from the manufacturer of the LCM device. Once optimized, quantified and approved by the user, the resulting outline of the region of interest is directly copied to the laser beam cutting trajectory. The software is made publicly available for non-commercial use to the scientific community and provides a versatile tool for effectively minimizing the length of the laser trajectory to obtain the selected cells without destroying surrounding cells and tissue structures. Saving all the settings allows for performing repeating tasks under similar conditions, especially in uniform and routinely performed LCM applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biologia

ISSN

0006-3088

e-ISSN

—

Svazek periodika

74

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

8

Strana od-do

717-724

Kód UT WoS článku

000467648500015

EID výsledku v databázi Scopus

2-s2.0-85065705281