Frequency of Mutations Associated with Resistance to First- and Second-Line Drugs in Multidrug Resistant Mycobacterium Tuberculosis Isolates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F20%3A10410202" target="_blank" >RIV/00216208:11140/20:10410202 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/20:43879031

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=26g-E55xnk" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=26g-E55xnk</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jgar.2020.03.013" target="_blank" >10.1016/j.jgar.2020.03.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Frequency of Mutations Associated with Resistance to First- and Second-Line Drugs in Multidrug Resistant Mycobacterium Tuberculosis Isolates

Popis výsledku v původním jazyce

INTRODUCTION: Tuberculosis (TB) is considered one of the most fatal diseases worldwide with an estimation of 10.1 million cases (WHO 2018). In this study, Whole genome sequencing (WGS) was used to perform genomic characterization of 40 Mycobacterium tuberculosis (M. tuberculosis) isolates from patients with different nationalities hospitalized in the Czech Republic. MATERIALS AND METHODS: Susceptibility testing for first line drugs was performed. DNA was sequenced using Illumina MiSeq platform. Spoligotypes Single Nucleotide Polymorphisms (SNPs) and mutations in antibiotic resistant genes were detected and phylogenetic analysis was performed. RESULTS: Samples showing phenotypic resistance to at least one drug were 12 to streptomycin, 11 to isoniazid, seven to rifampicin, six to ethambutol and five to pyrazinamide. Phenotypic and genotypic profiles didn&apos;t match in all cases suggesting the presence of novel mutation in some cases and low expression of resistant genes in others. The presented phylogeny enables the correct assignation of M. tuberculosis lineages and sub-lineages. Our results suggest that the most dominant lineage in our samples was lineages 4 (33/40). CONCLUSION: To our knowledge, this is the first study using this approach to be done in Czech Republic. Lineage 4 was the predominant lineage identified among our samples. Nevertheless, the dominance of Lineage 4 along with other lineages suggests that infections can be originated from different sources.

Název v anglickém jazyce

Frequency of Mutations Associated with Resistance to First- and Second-Line Drugs in Multidrug Resistant Mycobacterium Tuberculosis Isolates

Popis výsledku anglicky

INTRODUCTION: Tuberculosis (TB) is considered one of the most fatal diseases worldwide with an estimation of 10.1 million cases (WHO 2018). In this study, Whole genome sequencing (WGS) was used to perform genomic characterization of 40 Mycobacterium tuberculosis (M. tuberculosis) isolates from patients with different nationalities hospitalized in the Czech Republic. MATERIALS AND METHODS: Susceptibility testing for first line drugs was performed. DNA was sequenced using Illumina MiSeq platform. Spoligotypes Single Nucleotide Polymorphisms (SNPs) and mutations in antibiotic resistant genes were detected and phylogenetic analysis was performed. RESULTS: Samples showing phenotypic resistance to at least one drug were 12 to streptomycin, 11 to isoniazid, seven to rifampicin, six to ethambutol and five to pyrazinamide. Phenotypic and genotypic profiles didn&apos;t match in all cases suggesting the presence of novel mutation in some cases and low expression of resistant genes in others. The presented phylogeny enables the correct assignation of M. tuberculosis lineages and sub-lineages. Our results suggest that the most dominant lineage in our samples was lineages 4 (33/40). CONCLUSION: To our knowledge, this is the first study using this approach to be done in Czech Republic. Lineage 4 was the predominant lineage identified among our samples. Nevertheless, the dominance of Lineage 4 along with other lineages suggests that infections can be originated from different sources.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Global Antimicrobial Resistance

ISSN

2213-7165

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

275-282

Kód UT WoS článku

000572257300037

EID výsledku v databázi Scopus

2-s2.0-85087002151