Method for detection and identification of saccharides by mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F25%3A10504673" target="_blank" >RIV/00216208:11140/25:10504673 - isvavai.cz</a>

Výsledek na webu

<a href="https://patentscope.wipo.int/search/en/WO2025171832" target="_blank" >https://patentscope.wipo.int/search/en/WO2025171832</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Method for detection and identification of saccharides by mass spectrometry

Popis výsledku v původním jazyce

The present disclosure provides a method for the detection and/or identification of reducing monosaccharides or oligosaccharides by mass spectrometry in a sample, wherein the reducing saccharide(s) in the sample is (are) reacted with a ligand of general formula I (I), wherein L is selected from the group comprising, wherein n is from 1 to 3,,,, wherein m is 1 or 2, and, wherein in the general formula I the methoxyphenol group is bound to the carbon atom of the linker L, where R1, R2 and R4 are independently selected from the group comprising hydrogen atom and, wherein R3 is selected from the group comprising hydrogen atom and methyl, to form a conjugate by reaction of the aldehyde group(s) of the reducing monosaccharide(s) and oligosaccharide(s) present in the sample with the NH2 group of the ligand, and the conjugate is subsequently subjected to ionization and mass spectrometry measurement. This method can be used also for detection or identification of microbial polysaccharides by mass spectrometry, wherein prior to the reaction with the ligand, the polysaccharides are cleaved enzymatically or by acid hydrolysis to reducing monosaccharides and/or oligosaccharides.

Název v anglickém jazyce

Method for detection and identification of saccharides by mass spectrometry

Popis výsledku anglicky

The present disclosure provides a method for the detection and/or identification of reducing monosaccharides or oligosaccharides by mass spectrometry in a sample, wherein the reducing saccharide(s) in the sample is (are) reacted with a ligand of general formula I (I), wherein L is selected from the group comprising, wherein n is from 1 to 3,,,, wherein m is 1 or 2, and, wherein in the general formula I the methoxyphenol group is bound to the carbon atom of the linker L, where R1, R2 and R4 are independently selected from the group comprising hydrogen atom and, wherein R3 is selected from the group comprising hydrogen atom and methyl, to form a conjugate by reaction of the aldehyde group(s) of the reducing monosaccharide(s) and oligosaccharide(s) present in the sample with the NH2 group of the ligand, and the conjugate is subsequently subjected to ionization and mass spectrometry measurement. This method can be used also for detection or identification of microbial polysaccharides by mass spectrometry, wherein prior to the reaction with the ligand, the polysaccharides are cleaved enzymatically or by acid hydrolysis to reducing monosaccharides and/or oligosaccharides.

Druh

P - Patent

CEP obor

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OECD FORD obor

30303 - Infectious Diseases

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Číslo patentu nebo vzoru

PCT/CZ2025/050014

Vydavatel

EPO_1 -

Název vydavatele

European Patent Office

Místo vydání

Munich, The Hague, Berlin, Vienna, Brussels

Stát vydání

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Datum přijetí

9. 2. 2025

Název vlastníka

Univerzita Karlova

Způsob využití

B - Výsledek je využíván orgány státní nebo veřejné správy

Druh možnosti využití

A - K využití výsledku jiným subjektem je vždy nutné nabytí licence