Salicylát sodný inhibuje NF-kapaB a indukuje apoptózu v buňkách PC12

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F04%3A00004957" target="_blank" >RIV/00216208:11150/04:00004957 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sodium salicylate inhibits NF-kapaB and induces apoptosis in PC12 cells

Popis výsledku v původním jazyce

The main goals of this study were to demonstrate that inhibition of NF-kB by sodium salicylate decreases the viability of rat phaeochromocytoma PC12 cells and to investigate the nature of cell damage and death. PC12 cells were treated with different concentrations of sodium salicylate (1-20 mM). Higher concentrations (10-20 mM) killed PC12 cells in a dose-dependent manner. The assessments were done by direct cell counting in a Bürker chamber and by the WST-1 cytotoxicity assay. We also found a decreasedNF-kB activity after sodium salicylate treatment by electrophoretic mobility shift assay (EMSA). The cells treated with sodium salicylate were undergoing apoptosis as seen on our records obtained by time-lapse videomicroscopy as well as shown by DNA fragmentation experiments. The decreased DNA binding activity of NF-kB indicates that the inhibition of NF-kB can play a role in these processes.

Název v anglickém jazyce

Sodium salicylate inhibits NF-kapaB and induces apoptosis in PC12 cells

Popis výsledku anglicky

The main goals of this study were to demonstrate that inhibition of NF-kB by sodium salicylate decreases the viability of rat phaeochromocytoma PC12 cells and to investigate the nature of cell damage and death. PC12 cells were treated with different concentrations of sodium salicylate (1-20 mM). Higher concentrations (10-20 mM) killed PC12 cells in a dose-dependent manner. The assessments were done by direct cell counting in a Bürker chamber and by the WST-1 cytotoxicity assay. We also found a decreasedNF-kB activity after sodium salicylate treatment by electrophoretic mobility shift assay (EMSA). The cells treated with sodium salicylate were undergoing apoptosis as seen on our records obtained by time-lapse videomicroscopy as well as shown by DNA fragmentation experiments. The decreased DNA binding activity of NF-kB indicates that the inhibition of NF-kB can play a role in these processes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biochemical and Biophysical Methods

ISSN

0165-022X

e-ISSN

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Svazek periodika

61

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

229-240

Kód UT WoS článku

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EID výsledku v databázi Scopus

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