Ambivalentní účinky zinku na chromem (VI) vyvolaný oxidační stres a apoptózu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F05%3A00003630" target="_blank" >RIV/00216208:11150/05:00003630 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/05:00003634

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Zinc has ambiguous effects on chromium (VI)-induced oxidative stress and apoptosis

Popis výsledku v původním jazyce

Zinc is an important cellular antioxidant. We investigated its role in chromium-induced oxidative stress and apoptosis in human tumor cell line Hep-2. The measured parameters included intracellular labile zinc content (Zinquin-E fluorescence), cell viability (WST-1 assay), oxidative stress (spectrophotometry), mitochondrial potential (flow cytometry), caspase-3 activity, and PARP cleavage (immunofluoroscence). We found that Hep-2 cells contain abundant labile zinc stores that may be depleted by the ionophore TPEN or increased by external zinc supplementation. Chromium (VI)-induced cytotoxicity and apoptosis were enhanced in zinc-depleted cells after 24 h, in particular at chromium (VI) concentrations of 50 umol/l. On the other hand, elevated levels oflabile zinc were able to protect against apoptosis induced by 10 umol/l chromium (VI) but at higher chromium (VI) concentrations (50 and 150 umol/l) acted synergistically, significantly enhancing oxidative stress and the course of apoptos

Název v anglickém jazyce

Zinc has ambiguous effects on chromium (VI)-induced oxidative stress and apoptosis

Popis výsledku anglicky

Zinc is an important cellular antioxidant. We investigated its role in chromium-induced oxidative stress and apoptosis in human tumor cell line Hep-2. The measured parameters included intracellular labile zinc content (Zinquin-E fluorescence), cell viability (WST-1 assay), oxidative stress (spectrophotometry), mitochondrial potential (flow cytometry), caspase-3 activity, and PARP cleavage (immunofluoroscence). We found that Hep-2 cells contain abundant labile zinc stores that may be depleted by the ionophore TPEN or increased by external zinc supplementation. Chromium (VI)-induced cytotoxicity and apoptosis were enhanced in zinc-depleted cells after 24 h, in particular at chromium (VI) concentrations of 50 umol/l. On the other hand, elevated levels oflabile zinc were able to protect against apoptosis induced by 10 umol/l chromium (VI) but at higher chromium (VI) concentrations (50 and 150 umol/l) acted synergistically, significantly enhancing oxidative stress and the course of apoptos

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Trace Elements in Medicine and Biology

ISSN

0946-672X

e-ISSN

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Svazek periodika

18

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

251-260

Kód UT WoS článku

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EID výsledku v databázi Scopus

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