Protektivní účinek S-ademosylmethioninu na buněčné a mitochondriální membráně potkaních hepatocytů proti tert-butylhydroperoxidem navozenému poškození v primární kultuře

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F05%3A00003640" target="_blank" >RIV/00216208:11150/05:00003640 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216275:25310/05:00006080

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Protective effect of S-adenosylmethionine on cellular and mitochondrial membranes of rat hepatocytes against tert-butylhydroperoxide-induced injury in primary culture

Popis výsledku v původním jazyce

We evaluated the effect of SAMe on hepatocyte injury induced in culture by oxidant substance tert-butylhydroperoxide(1.5 mM tBHP) with regard to prevent mitochondrial injury.The pretreatment of hepatocyte culture with SAMe in doses of 0.25,0.5,1,2.5,5,10,25 and 50 mg/l for 30 minutes prevented the release of LDH from cells incubated for 30 minutes with tBHP in a dose dependent manner.The inhibitory effect of SAMe on lipid peroxidation paralled the effect on cell viability.SAMe also moderated the decrease of the mitochondrial membrane potential induced by tBHP.Our results indicate that the inhibition of lipid peroxidation by SAMe can contribute to the prevention of disruption of both cellular and mitochondrial membranes.While the protective effect of SAMe against tBHP-induced GSH depletion was not confirmed,probably the most potent effect of SAMe on membranes by phospholipid methylation should be verified.

Název v anglickém jazyce

Protective effect of S-adenosylmethionine on cellular and mitochondrial membranes of rat hepatocytes against tert-butylhydroperoxide-induced injury in primary culture

Popis výsledku anglicky

We evaluated the effect of SAMe on hepatocyte injury induced in culture by oxidant substance tert-butylhydroperoxide(1.5 mM tBHP) with regard to prevent mitochondrial injury.The pretreatment of hepatocyte culture with SAMe in doses of 0.25,0.5,1,2.5,5,10,25 and 50 mg/l for 30 minutes prevented the release of LDH from cells incubated for 30 minutes with tBHP in a dose dependent manner.The inhibitory effect of SAMe on lipid peroxidation paralled the effect on cell viability.SAMe also moderated the decrease of the mitochondrial membrane potential induced by tBHP.Our results indicate that the inhibition of lipid peroxidation by SAMe can contribute to the prevention of disruption of both cellular and mitochondrial membranes.While the protective effect of SAMe against tBHP-induced GSH depletion was not confirmed,probably the most potent effect of SAMe on membranes by phospholipid methylation should be verified.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemico-Biological Interactions

ISSN

0009-2797

e-ISSN

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Svazek periodika

156

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

13-23

Kód UT WoS článku

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EID výsledku v databázi Scopus

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