Model poškození primární kultury hepatocytů potkana D-galaktosaminem
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F06%3A00004180" target="_blank" >RIV/00216208:11150/06:00004180 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216275:25310/06:00006539
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The model of D-galactosamine-induced injury of rat hepatocytes in primary culture
Popis výsledku v původním jazyce
D-galactosamine (GaIN) is a highly selective hepatotoxin that causes liver damage similar to viral hepatitis via depletion of uridine nucleotides, which subsequently diminishes synthesis of RNA and proteins. Model of galactosamine heaptotoxcity is frequently used in animal experiments in vivo. The purpose of our study was to establish the model of GaIN-induced hepatocyte injury in in vitro conditions using primocultures of rat hepatocytes as an important prerequisite for furhter experiments in which wewould like to study potential hepatoprotective effect of various substances. Rate of heaptocyte injury was evaluated by morphological changes, changes in cell viability, albumin production, mitochondrial membrane potential, activity of mitcohondrial dehydrogenases and glutathione content. Marked dose dependent hepatocyte imjury was found after 24-hour incubation with GaIN. Based on the results we suggest as an optimal model for short-term toxciity test exposure to GaIN for 24 hours in do
Název v anglickém jazyce
The model of D-galactosamine-induced injury of rat hepatocytes in primary culture
Popis výsledku anglicky
D-galactosamine (GaIN) is a highly selective hepatotoxin that causes liver damage similar to viral hepatitis via depletion of uridine nucleotides, which subsequently diminishes synthesis of RNA and proteins. Model of galactosamine heaptotoxcity is frequently used in animal experiments in vivo. The purpose of our study was to establish the model of GaIN-induced hepatocyte injury in in vitro conditions using primocultures of rat hepatocytes as an important prerequisite for furhter experiments in which wewould like to study potential hepatoprotective effect of various substances. Rate of heaptocyte injury was evaluated by morphological changes, changes in cell viability, albumin production, mitochondrial membrane potential, activity of mitcohondrial dehydrogenases and glutathione content. Marked dose dependent hepatocyte imjury was found after 24-hour incubation with GaIN. Based on the results we suggest as an optimal model for short-term toxciity test exposure to GaIN for 24 hours in do
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FB - Endokrinologie, diabetologie, metabolismus, výživa
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2006
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Acta Medica (Hradec Králové)
ISSN
1211-4286
e-ISSN
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Svazek periodika
49
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
7
Strana od-do
59-65
Kód UT WoS článku
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EID výsledku v databázi Scopus
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