Metabolismus proteinů v pomalých a rychlých vláknech kosterního svalu během zánětu vyvolaného aplikací terpentýnu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F07%3A00003721" target="_blank" >RIV/00216208:11150/07:00003721 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/08:00109101

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Protein metabolism in slow- and fast-twitch skeletal muscle during turpentine-induced inflammation

Popis výsledku v původním jazyce

The aim of our study was to evaluate the differences in protein and amino acid metabolism in the soleus (SOL) and the extensor digitorum longus muscle (EDL) after turpentine administration.Young rats were injected subcutaneously with turpentine oil (inflammation) or with saline solution (control).Twenty-four hours later muscles were dissected and incubated in modified Krebs-Heinseleit buffer to estimate total and myofibrillar proteolysis,chymotrypsin-like activity of proteasome (CHTLA),leucine oxidation,protein synthesis and amino acid release into the medium.The data obtained demonstrate that in intact rats,all parameters measured except protein synthesis are significantly higher in SOL than in EDL.In turpentine treated animals,CHTLA increaed and protein synthesis decreased significantly more in EDL.Release of leucine was inhibitied significantly more in SOL.We conclude that turpentine induced inflammation affects more CHTLA,protein synthesis and leucine release in EDL compared to SOL

Název v anglickém jazyce

Protein metabolism in slow- and fast-twitch skeletal muscle during turpentine-induced inflammation

Popis výsledku anglicky

The aim of our study was to evaluate the differences in protein and amino acid metabolism in the soleus (SOL) and the extensor digitorum longus muscle (EDL) after turpentine administration.Young rats were injected subcutaneously with turpentine oil (inflammation) or with saline solution (control).Twenty-four hours later muscles were dissected and incubated in modified Krebs-Heinseleit buffer to estimate total and myofibrillar proteolysis,chymotrypsin-like activity of proteasome (CHTLA),leucine oxidation,protein synthesis and amino acid release into the medium.The data obtained demonstrate that in intact rats,all parameters measured except protein synthesis are significantly higher in SOL than in EDL.In turpentine treated animals,CHTLA increaed and protein synthesis decreased significantly more in EDL.Release of leucine was inhibitied significantly more in SOL.We conclude that turpentine induced inflammation affects more CHTLA,protein synthesis and leucine release in EDL compared to SOL

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Experimental Pathology

ISSN

0959-9673

e-ISSN

—

Svazek periodika

89

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

64-71

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—