Exprese of mRNA, které mají vztah k metabolismu pojivové tkáně, v jaterních hvězdicových buňkách a myofibroblastech

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F07%3A00004289" target="_blank" >RIV/00216208:11150/07:00004289 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/07:00001802

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts

Popis výsledku v původním jazyce

Hepatic stellate cells (HSC) and liver myofibroblasts (MFB) are two cell populations most likely responsible for the synthesis of most connective tissue components in fibrotic liver. Normal and carbon tetrachloride-cirrhotic livers from rats were used toprepare HSC. Liver was perfused with pronase and collagenase solutions, followed by centrifugation of the cell suspension on a density gradient. HSC were quiescent 2 days after plating on plastic but they became activated after another 5 days in culture. When the culture was passaged 5 times, its character changed profoundly as HSC were replaced by MFB. Microarray analysis was used to determine gene expression in quiescent HSC, activated HSC and MFB. The expression of 49 genes coding for connective tissue proteins, proteoglycans, metalloproteinases and their inhibitors, growth factors and cellular markers was determined in quiescent HSC, activated HSC and MFB.

Název v anglickém jazyce

Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts

Popis výsledku anglicky

Hepatic stellate cells (HSC) and liver myofibroblasts (MFB) are two cell populations most likely responsible for the synthesis of most connective tissue components in fibrotic liver. Normal and carbon tetrachloride-cirrhotic livers from rats were used toprepare HSC. Liver was perfused with pronase and collagenase solutions, followed by centrifugation of the cell suspension on a density gradient. HSC were quiescent 2 days after plating on plastic but they became activated after another 5 days in culture. When the culture was passaged 5 times, its character changed profoundly as HSC were replaced by MFB. Microarray analysis was used to determine gene expression in quiescent HSC, activated HSC and MFB. The expression of 49 genes coding for connective tissue proteins, proteoglycans, metalloproteinases and their inhibitors, growth factors and cellular markers was determined in quiescent HSC, activated HSC and MFB.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

<a href="/cs/project/GA305%2F03%2F1513" target="_blank" >GA305/03/1513: Exprese vazivových bílkovin v jaterních lipocytech potkanů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental and Toxicological Pathology

ISSN

0940-2993

e-ISSN

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Svazek periodika

58

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

263-273

Kód UT WoS článku

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EID výsledku v databázi Scopus

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