Antiproliferative and cytotoxic effects of sodium selenite in human colon cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F09%3A00208736" target="_blank" >RIV/00216208:11150/09:00208736 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Antiproliferative and cytotoxic effects of sodium selenite in human colon cancer cells

Popis výsledku v původním jazyce

We evaluated the antiproliferative potential of sodium selenite in HCT-116 colorectal cells with wild-type p53 and its isogenic control HCT-116-p53KO cell line. Cell proliferation was followed by time-lapse videomicroscopy, by measuring protein content ,metabolic activity and DNA synthesis. Changes in cell cycle were determined by flow cytometry and Western blotting. Cell death was measured with the nuclear fragmentation assay and caspase-3 immunostaining. Sodium selenite inhibits proliferation of colon cancer cells in a time- and dose-dependent manner, with HCT-116 cells being more sensitive than HCT-116-p53KO cells. There was a tendency for cells to accumulate at G2 phase which was accompanied by the increasing expression of cyclin B1, Cdc2 p34, p21and the sub G1 fraction of the cell cycle. In addition, PARP and nuclear fragmentation and activation of caspase-3 were more profound in HCT-116 cells versus HCT-116-p53KO cells, indicating important role of p53 in Se-induced toxicity.

Název v anglickém jazyce

Antiproliferative and cytotoxic effects of sodium selenite in human colon cancer cells

Popis výsledku anglicky

We evaluated the antiproliferative potential of sodium selenite in HCT-116 colorectal cells with wild-type p53 and its isogenic control HCT-116-p53KO cell line. Cell proliferation was followed by time-lapse videomicroscopy, by measuring protein content ,metabolic activity and DNA synthesis. Changes in cell cycle were determined by flow cytometry and Western blotting. Cell death was measured with the nuclear fragmentation assay and caspase-3 immunostaining. Sodium selenite inhibits proliferation of colon cancer cells in a time- and dose-dependent manner, with HCT-116 cells being more sensitive than HCT-116-p53KO cells. There was a tendency for cells to accumulate at G2 phase which was accompanied by the increasing expression of cyclin B1, Cdc2 p34, p21and the sub G1 fraction of the cell cycle. In addition, PARP and nuclear fragmentation and activation of caspase-3 were more profound in HCT-116 cells versus HCT-116-p53KO cells, indicating important role of p53 in Se-induced toxicity.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology in Vitro

ISSN

0887-2333

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

8,SpSup

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000272276600010

EID výsledku v databázi Scopus

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