Glutathione Reductase Is Inhibited by Acetaminophen-glutathione Conjugate In Vitro
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F10%3A10080783" target="_blank" >RIV/00216208:11150/10:10080783 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216275:25310/10:39883232
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Glutathione Reductase Is Inhibited by Acetaminophen-glutathione Conjugate In Vitro
Popis výsledku v původním jazyce
The aim of the present work was to investigate a new mechanism likely contributing to the toxic action of acetaminophen especially to explore the possible inhibition of glutathione reductase through an acetaminophen-glutathione conjugate (APAP-SG). APAP-SG conjugate was synthesized by organic synthesis and purified by column chromatography. The inhibitory effect of the conjugate on two types of glutathione reductase (from yeasts and rat hepatocytes) was tested spectrophotometrically. In addition, the enzyme acitivty (from hepatocytes lysate) was decreased to 79?7%, 67?2% and 39?7%, in 0.37, 1.48 and 3.7 mM concentration of the conjugate, respecitvely. We ound that glutathione reductase, the essential enzyme of the antioxidant system, was dosedependently inhibited by the product of acetaminophen metabolism - the conjugate of acetaminophen and glutathione.
Název v anglickém jazyce
Glutathione Reductase Is Inhibited by Acetaminophen-glutathione Conjugate In Vitro
Popis výsledku anglicky
The aim of the present work was to investigate a new mechanism likely contributing to the toxic action of acetaminophen especially to explore the possible inhibition of glutathione reductase through an acetaminophen-glutathione conjugate (APAP-SG). APAP-SG conjugate was synthesized by organic synthesis and purified by column chromatography. The inhibitory effect of the conjugate on two types of glutathione reductase (from yeasts and rat hepatocytes) was tested spectrophotometrically. In addition, the enzyme acitivty (from hepatocytes lysate) was decreased to 79?7%, 67?2% and 39?7%, in 0.37, 1.48 and 3.7 mM concentration of the conjugate, respecitvely. We ound that glutathione reductase, the essential enzyme of the antioxidant system, was dosedependently inhibited by the product of acetaminophen metabolism - the conjugate of acetaminophen and glutathione.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
ED - Fyziologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Physiological Research
ISSN
0862-8408
e-ISSN
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Svazek periodika
59
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
6
Strana od-do
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Kód UT WoS článku
000278016200009
EID výsledku v databázi Scopus
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