Role of Transplanted Bone Marrow Cells in Response to Skeletal Muscle Injury
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F11%3A10104934" target="_blank" >RIV/00216208:11150/11:10104934 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60162694:G44__/11:00002607
Výsledek na webu
<a href="http://fb.cuni.cz/file/5610/FB2011A0033.pdf" target="_blank" >http://fb.cuni.cz/file/5610/FB2011A0033.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Role of Transplanted Bone Marrow Cells in Response to Skeletal Muscle Injury
Popis výsledku v původním jazyce
In this work, recipient mice were intramuscularly injected with cardiotoxin, then whole-body lethally irradiated to eradicate satellite cells in their injured tibialis anterior muscles and to suppress hematopoiesis and subsequently, intravenously transplanted with mouse donor lacZ+ bone marrow cells with the aim to investigate role of exogenous bone marrow cells in response to skeletal muscle injury. Seven to 33 days after grafting, recipient tibialis anterior muscles were examined to detect donor-derived X-gal+ cells and analyzed by quantitative PCR. In injured recipients'' muscles, X-gal positivity was identified 14 and 33 days after grafting in some infiltrating neutrophils and macrophages, infrequently in fibroblasts of endomysium and in many largemultinucleated cells (devoid of myogenic markers desmin and nestin) resembling foreign body giant cells situated in vicinity to necrotic muscle fibers. qPCR confirmed the presence of lacZ+ cells in injured recipients'' muscles. Our resul
Název v anglickém jazyce
Role of Transplanted Bone Marrow Cells in Response to Skeletal Muscle Injury
Popis výsledku anglicky
In this work, recipient mice were intramuscularly injected with cardiotoxin, then whole-body lethally irradiated to eradicate satellite cells in their injured tibialis anterior muscles and to suppress hematopoiesis and subsequently, intravenously transplanted with mouse donor lacZ+ bone marrow cells with the aim to investigate role of exogenous bone marrow cells in response to skeletal muscle injury. Seven to 33 days after grafting, recipient tibialis anterior muscles were examined to detect donor-derived X-gal+ cells and analyzed by quantitative PCR. In injured recipients'' muscles, X-gal positivity was identified 14 and 33 days after grafting in some infiltrating neutrophils and macrophages, infrequently in fibroblasts of endomysium and in many largemultinucleated cells (devoid of myogenic markers desmin and nestin) resembling foreign body giant cells situated in vicinity to necrotic muscle fibers. qPCR confirmed the presence of lacZ+ cells in injured recipients'' muscles. Our resul
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EA - Morfologické obory a cytologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Folia Biologica
ISSN
0015-5500
e-ISSN
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Svazek periodika
57
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
10
Strana od-do
232-241
Kód UT WoS článku
000299144400002
EID výsledku v databázi Scopus
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