Prominin-1 (CD133) is not restricted to stem cells located in the basal compartment of murine and human prostate

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F11%3A10109261" target="_blank" >RIV/00216208:11150/11:10109261 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/pros.21239/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/pros.21239/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/pros.21239" target="_blank" >10.1002/pros.21239</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Prominin-1 (CD133) is not restricted to stem cells located in the basal compartment of murine and human prostate

Popis výsledku v původním jazyce

Rodent and human prominin-1 are expressed in numerous adult epithelia and somatic stem cells. Previous report has shown that human PROMININ-1 carrying the AC133 epitope can be used to identify rare prostate basal stem cells (Richardson et al., J Cell Sci2004; 117:3539-3545). Here we re-investigated its general expression in male reproductive tract including mouse and human prostate and in prostate cancer samples using various anti-prominin-1 antibodies. Murine tissues were stained with 13A4 monoclonalantibody (mAb) whereas human samples were examined either with the AC133 mAb recognizing the AC133 glycosylation-dependent epitope or 80B258 mAb directed against the PROMININ-1 polypeptide. Mouse prominin-1 was detected at the apical domain of epithelialcells of ductus deferens, seminal vesicles, ampullary glands, and all prostatic lobes. In human prostate, immunoreactivity for 80B258, but not AC133 was revealed at the apical side of some epithelial (luminal) cells, in addition to the m

Název v anglickém jazyce

Prominin-1 (CD133) is not restricted to stem cells located in the basal compartment of murine and human prostate

Popis výsledku anglicky

Rodent and human prominin-1 are expressed in numerous adult epithelia and somatic stem cells. Previous report has shown that human PROMININ-1 carrying the AC133 epitope can be used to identify rare prostate basal stem cells (Richardson et al., J Cell Sci2004; 117:3539-3545). Here we re-investigated its general expression in male reproductive tract including mouse and human prostate and in prostate cancer samples using various anti-prominin-1 antibodies. Murine tissues were stained with 13A4 monoclonalantibody (mAb) whereas human samples were examined either with the AC133 mAb recognizing the AC133 glycosylation-dependent epitope or 80B258 mAb directed against the PROMININ-1 polypeptide. Mouse prominin-1 was detected at the apical domain of epithelialcells of ductus deferens, seminal vesicles, ampullary glands, and all prostatic lobes. In human prostate, immunoreactivity for 80B258, but not AC133 was revealed at the apical side of some epithelial (luminal) cells, in addition to the m

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Prostate

ISSN

0270-4137

e-ISSN

—

Svazek periodika

71

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

254-267

Kód UT WoS článku

000287059600004

EID výsledku v databázi Scopus

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