The dose-dependent effects of endotoxin on protein metabolism in two types of rat skeletal muscle

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10124769" target="_blank" >RIV/00216208:11150/12:10124769 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/12:10124769

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs13105-012-0150-6" target="_blank" >http://link.springer.com/article/10.1007%2Fs13105-012-0150-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13105-012-0150-6" target="_blank" >10.1007/s13105-012-0150-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The dose-dependent effects of endotoxin on protein metabolism in two types of rat skeletal muscle

Popis výsledku v původním jazyce

The main purpose of this study was determining the effect of various doses of endotoxin on protein and amino acid metabolism in two types of rat skeletal muscle. Sepsis was induced by intraperitoneal administration of endotoxin in a dose of 1, 3 and 5 mg/kg body weithg (bw). After 24 h, extensor digitorum longus (EDL) and soleus (SOL) muscles were isolated and used for determination of total and myofibrillar proteolysis, protein synthesis, activity of cathepsins B and L, chymotrypsin-like activity of proteasome and amino acid release. The dose of 5 mg of endotoxin/kg bw induced the most significant changes in parameters of the protein and amino acid metabolism measured and could be therefore considered appropriate for studies of protein catabolism in young rat skeletal muscle at 24 h after endotoxin treatment.

Název v anglickém jazyce

The dose-dependent effects of endotoxin on protein metabolism in two types of rat skeletal muscle

Popis výsledku anglicky

The main purpose of this study was determining the effect of various doses of endotoxin on protein and amino acid metabolism in two types of rat skeletal muscle. Sepsis was induced by intraperitoneal administration of endotoxin in a dose of 1, 3 and 5 mg/kg body weithg (bw). After 24 h, extensor digitorum longus (EDL) and soleus (SOL) muscles were isolated and used for determination of total and myofibrillar proteolysis, protein synthesis, activity of cathepsins B and L, chymotrypsin-like activity of proteasome and amino acid release. The dose of 5 mg of endotoxin/kg bw induced the most significant changes in parameters of the protein and amino acid metabolism measured and could be therefore considered appropriate for studies of protein catabolism in young rat skeletal muscle at 24 h after endotoxin treatment.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physiology and Biochemistry

ISSN

1138-7548

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

ES - Španělské království

Počet stran výsledku

11

Strana od-do

385-395

Kód UT WoS článku

000307513500009

EID výsledku v databázi Scopus

—