Determination of pravastatin and pravastatin lactone in rat plasma and urine using UHPLC-MS/MS and microextraction by packed sorbent

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10124845" target="_blank" >RIV/00216208:11150/12:10124845 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/12:10124845

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0039914011011118" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0039914011011118</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2011.12.043" target="_blank" >10.1016/j.talanta.2011.12.043</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of pravastatin and pravastatin lactone in rat plasma and urine using UHPLC-MS/MS and microextraction by packed sorbent

Popis výsledku v původním jazyce

A simple and reproducible method for the determination of pravastatin and pravastatin lactone in rat plasma and urine by means of ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) using deuterium labeled intemal standardsfor quantification is reported. Separation of analytes was performed on BEH C-18 analytical column (50 mm x 2.1 mm, 1.7 mu m), using gradient elution by mobile phase consisting of acetonitrile and 1 mM ammonium acetate at pH 4.0. Run time was 2 min. Quantification of analytes was performed using the SRM (selected reaction monitoring) experiment in ESI negative ion mode for pravastatin and in ESI positive ion mode for pravastatin lactone. Sample treatment consisted of a protein precipitation by ACN andmicroextraction by packed sorbent (MEPS) for rat plasma. Simple MEPS procedure was sufficient for rat urine. MEPS was implemented using the C8 sorbent inserted into a microvolume syringe, eVol hand-held automated analytical syringe and a

Název v anglickém jazyce

Determination of pravastatin and pravastatin lactone in rat plasma and urine using UHPLC-MS/MS and microextraction by packed sorbent

Popis výsledku anglicky

A simple and reproducible method for the determination of pravastatin and pravastatin lactone in rat plasma and urine by means of ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) using deuterium labeled intemal standardsfor quantification is reported. Separation of analytes was performed on BEH C-18 analytical column (50 mm x 2.1 mm, 1.7 mu m), using gradient elution by mobile phase consisting of acetonitrile and 1 mM ammonium acetate at pH 4.0. Run time was 2 min. Quantification of analytes was performed using the SRM (selected reaction monitoring) experiment in ESI negative ion mode for pravastatin and in ESI positive ion mode for pravastatin lactone. Sample treatment consisted of a protein precipitation by ACN andmicroextraction by packed sorbent (MEPS) for rat plasma. Simple MEPS procedure was sufficient for rat urine. MEPS was implemented using the C8 sorbent inserted into a microvolume syringe, eVol hand-held automated analytical syringe and a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

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Svazek periodika

90

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

22-29

Kód UT WoS článku

000301627300003

EID výsledku v databázi Scopus

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