Activation of p38 MAPK and expression of TGF-beta 1 in rat colon enterocytes after whole body gamma-irradiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10125351" target="_blank" >RIV/00216208:11150/12:10125351 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/12:43874677

Výsledek na webu

<a href="http://informahealthcare.com/doi/pdf/10.3109/09553002.2012.654044" target="_blank" >http://informahealthcare.com/doi/pdf/10.3109/09553002.2012.654044</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3109/09553002.2012.654044" target="_blank" >10.3109/09553002.2012.654044</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Activation of p38 MAPK and expression of TGF-beta 1 in rat colon enterocytes after whole body gamma-irradiation

Popis výsledku v původním jazyce

Purpose : To examine the p38 mitogen-activated protein kinase (p38) phosphorylation and transforming growth factor beta 1 (TGF-beta 1) expression in rat colon enterocytes after irradiation and their contribution to pathology of intestinal radiation disease. Materials and methods : Male Wistar rats were irradiated with whole body gamma-radiation of 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 Gy (Co-60, 1.44 Gy.min(-1)). Samples were taken 4 and 24 h after irradiation, immunohistochemically stained, then p38 phosphorylation and TGF-beta 1 expression were measured in apical and cryptal enterocytes using computer image analysis. In selected groups, morphometric parameters, mitosis and apoptosis were evaluated. Results : P38 phosphorylation integrated optical density (IOD)-based levels increased 2.4-fold (p {= 0.01) and 3.6 to 22.8-fold (p {= 0.001) in apical enterocytes 4 h after 0.5 Gy and 24 h after 3-10 Gy, respectively. TGF-beta 1 IOD-based expression increased 3.3- to 6.9-fold (p {= 0.001)

Název v anglickém jazyce

Activation of p38 MAPK and expression of TGF-beta 1 in rat colon enterocytes after whole body gamma-irradiation

Popis výsledku anglicky

Purpose : To examine the p38 mitogen-activated protein kinase (p38) phosphorylation and transforming growth factor beta 1 (TGF-beta 1) expression in rat colon enterocytes after irradiation and their contribution to pathology of intestinal radiation disease. Materials and methods : Male Wistar rats were irradiated with whole body gamma-radiation of 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 Gy (Co-60, 1.44 Gy.min(-1)). Samples were taken 4 and 24 h after irradiation, immunohistochemically stained, then p38 phosphorylation and TGF-beta 1 expression were measured in apical and cryptal enterocytes using computer image analysis. In selected groups, morphometric parameters, mitosis and apoptosis were evaluated. Results : P38 phosphorylation integrated optical density (IOD)-based levels increased 2.4-fold (p {= 0.01) and 3.6 to 22.8-fold (p {= 0.001) in apical enterocytes 4 h after 0.5 Gy and 24 h after 3-10 Gy, respectively. TGF-beta 1 IOD-based expression increased 3.3- to 6.9-fold (p {= 0.001)

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/OVUOFVZ200812" target="_blank" >OVUOFVZ200812: RADSPEC - Krátkodobé a dlouhodobé nespecifické změny organismu exponovaného vysokým i nízkým dávkám nervově paralytických látek na buněčné a molekulární úrovni</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Radiation Biology

ISSN

0955-3002

e-ISSN

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Svazek periodika

88

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

348-358

Kód UT WoS článku

000302163000007

EID výsledku v databázi Scopus

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