Sulforaphane-induced apoptosis involves p53 and p38 in melanoma cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F14%3A10196731" target="_blank" >RIV/00216208:11150/14:10196731 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs10495-013-0959-7" target="_blank" >http://link.springer.com/article/10.1007%2Fs10495-013-0959-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10495-013-0959-7" target="_blank" >10.1007/s10495-013-0959-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sulforaphane-induced apoptosis involves p53 and p38 in melanoma cells

Popis výsledku v původním jazyce

In malignant melanoma complex reprogramming of cell death and survival pathways leads to increased chemoristance and poor longer-term survival. Sulforaphane (SF) is a promising isothiocyanate compound accurring in cruciferous plants with reported antiproliferative and proapoptotic activity in several tumor cell lines including melanoma. In this work we investigated the effects of SF in several melanoma cell lines and fresh melanoma cultivates. We found that SF is cytotoxic and induces mitochondrial, caspase-dependent apoptosis in our study model, however with lower efficiency in fresh melanoma cultivates. Moreover, our rsults indicate that in melanoma cell lines and fresh melanoma cultivates SF induces multiple signaling including oxidative stress-mediated activation of DNA-damage response pathway, changes in p38 kinase activity and enhanced expression of Bax and Puma proapoptotic proteins. In addition, in SF-exposed p53-mutant melanoma cells Puma expression seem to be under p38 contro

Název v anglickém jazyce

Sulforaphane-induced apoptosis involves p53 and p38 in melanoma cells

Popis výsledku anglicky

In malignant melanoma complex reprogramming of cell death and survival pathways leads to increased chemoristance and poor longer-term survival. Sulforaphane (SF) is a promising isothiocyanate compound accurring in cruciferous plants with reported antiproliferative and proapoptotic activity in several tumor cell lines including melanoma. In this work we investigated the effects of SF in several melanoma cell lines and fresh melanoma cultivates. We found that SF is cytotoxic and induces mitochondrial, caspase-dependent apoptosis in our study model, however with lower efficiency in fresh melanoma cultivates. Moreover, our rsults indicate that in melanoma cell lines and fresh melanoma cultivates SF induces multiple signaling including oxidative stress-mediated activation of DNA-damage response pathway, changes in p38 kinase activity and enhanced expression of Bax and Puma proapoptotic proteins. In addition, in SF-exposed p53-mutant melanoma cells Puma expression seem to be under p38 contro

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Apoptosis : an international journal on programmed cell death

ISSN

1360-8185

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

734-747

Kód UT WoS článku

000332485700015

EID výsledku v databázi Scopus

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