A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F15%3A10320056" target="_blank" >RIV/00216208:11150/15:10320056 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038" target="_blank" >http://dx.doi.org/10.1038</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/sdata.2015.46" target="_blank" >10.1038/sdata.2015.46</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development

Popis výsledku v původním jazyce

Resurgent interest in synaptic circuitry and plasticity has emphasized the importance of 3D reconstruction from serial section electron microscopy (3DEM). Three volumes of hippocampal CA1 neuropil from adult rat were imaged at X-Y resolution of ~2 nm onserial sections of ~50-60 nm thickness. These are the first densely reconstructed hippocampal volumes. All axons, dendrites, glia, and synapses were reconstructed in a cube (~10 ?m(3)) surrounding a large dendritic spine, a cylinder (~43 ?m(3)) surrounding an oblique dendritic segment (3.4 ?m long), and a parallelepiped (~178 ?m(3)) surrounding an apical dendritic segment (4.9 ?m long). The data provide standards for identifying ultrastructural objects in 3DEM, realistic reconstructions for modeling biophysical properties of synaptic transmission, and a test bed for enhancing reconstruction tools. Representative synapses are quantified from varying section planes, and microtubules, polyribosomes, smooth endoplasmic reticulum, and endoso

Název v anglickém jazyce

A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development

Popis výsledku anglicky

Resurgent interest in synaptic circuitry and plasticity has emphasized the importance of 3D reconstruction from serial section electron microscopy (3DEM). Three volumes of hippocampal CA1 neuropil from adult rat were imaged at X-Y resolution of ~2 nm onserial sections of ~50-60 nm thickness. These are the first densely reconstructed hippocampal volumes. All axons, dendrites, glia, and synapses were reconstructed in a cube (~10 ?m(3)) surrounding a large dendritic spine, a cylinder (~43 ?m(3)) surrounding an oblique dendritic segment (3.4 ?m long), and a parallelepiped (~178 ?m(3)) surrounding an apical dendritic segment (4.9 ?m long). The data provide standards for identifying ultrastructural objects in 3DEM, realistic reconstructions for modeling biophysical properties of synaptic transmission, and a test bed for enhancing reconstruction tools. Representative synapses are quantified from varying section planes, and microtubules, polyribosomes, smooth endoplasmic reticulum, and endoso

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Data [online]

ISSN

2052-4463

e-ISSN

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Svazek periodika

2

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

19

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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