Antiproliferative effects of α-tomatine are associated with different cell death modalities in human colon cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F16%3A10328502" target="_blank" >RIV/00216208:11150/16:10328502 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S1756464616303188" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1756464616303188</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jff.2016.10.005" target="_blank" >10.1016/j.jff.2016.10.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antiproliferative effects of α-tomatine are associated with different cell death modalities in human colon cancer cells

Popis výsledku v původním jazyce

Antitumour effects of α-tomatine were studied during 24 hours in a panel of human colon cancer cells. α-Tomatine proved to induce dose-dependent cytotoxicity resulting in morphologically varying cell demise with predominantly occurring necrotic phenotypes and minor presence of cells dying via caspase-independent apoptosis. Analyses into the nature of mechanisms responsible for activation of cell death revealed that following α-tomatine treatment, changes in RIP3 and RIP1 protein expression take place along with cyclophilin D mitochondrial accumulation. Moreover, in treated cells, lysosomal membrane permeabilization as well as mitochondrial perturbation with subsequent mitochondrial release of apoptosis-inducing factor (AIF) contributes to the execution of diverse death phenotypes possibly via enhanced activity JNK but in the absence of significant oxidative stress. Thus α-tomatine exhibits significant antitumour activity in colon cancer cells via targeting their membranous compartments and inducing both necroptosis and apoptosis.

Název v anglickém jazyce

Antiproliferative effects of α-tomatine are associated with different cell death modalities in human colon cancer cells

Popis výsledku anglicky

Antitumour effects of α-tomatine were studied during 24 hours in a panel of human colon cancer cells. α-Tomatine proved to induce dose-dependent cytotoxicity resulting in morphologically varying cell demise with predominantly occurring necrotic phenotypes and minor presence of cells dying via caspase-independent apoptosis. Analyses into the nature of mechanisms responsible for activation of cell death revealed that following α-tomatine treatment, changes in RIP3 and RIP1 protein expression take place along with cyclophilin D mitochondrial accumulation. Moreover, in treated cells, lysosomal membrane permeabilization as well as mitochondrial perturbation with subsequent mitochondrial release of apoptosis-inducing factor (AIF) contributes to the execution of diverse death phenotypes possibly via enhanced activity JNK but in the absence of significant oxidative stress. Thus α-tomatine exhibits significant antitumour activity in colon cancer cells via targeting their membranous compartments and inducing both necroptosis and apoptosis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Functional Foods

ISSN

1756-4646

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

491-502

Kód UT WoS článku

000390180000043

EID výsledku v databázi Scopus

2-s2.0-84994043931