Cloning of intronic sequence within DsRed2 increased the number of cells expressing red fluorescent protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F17%3A10368707" target="_blank" >RIV/00216208:11150/17:10368707 - isvavai.cz</a>

Výsledek na webu

<a href="http://biomed.papers.upol.cz/pdfs/bio/2017/04/04.pdf" target="_blank" >http://biomed.papers.upol.cz/pdfs/bio/2017/04/04.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2017.033" target="_blank" >10.5507/bp.2017.033</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cloning of intronic sequence within DsRed2 increased the number of cells expressing red fluorescent protein

Popis výsledku v původním jazyce

Aim. Cloning of artificial intronic sequence within the open reading frame (ORF) of DsRed2 gene. Method. Splice prediction software was used to analyze DsRed2 sequence to find an ideal site for cloning artificial intronic sequence. Intron was cloned within DsRed2 using cyclic ligation assembly. Flow cytometry was used to quantify the number of cells expressing red fluorescence. Result. Sequencing data confirmed precise cloning of intron at the desired position using cyclic ligation assembly. Successful expression of red fluorescence after cloning of intron confirmed successful intron recognition and splicing by host cell line. Cloning of intron increased the number of cells expressing red fluorescent protein. Conclusion. Cloning of intronic sequence within DsRed2 has helped to increase the number of cells expressing red fluorescence by approximately four percent.

Název v anglickém jazyce

Cloning of intronic sequence within DsRed2 increased the number of cells expressing red fluorescent protein

Popis výsledku anglicky

Aim. Cloning of artificial intronic sequence within the open reading frame (ORF) of DsRed2 gene. Method. Splice prediction software was used to analyze DsRed2 sequence to find an ideal site for cloning artificial intronic sequence. Intron was cloned within DsRed2 using cyclic ligation assembly. Flow cytometry was used to quantify the number of cells expressing red fluorescence. Result. Sequencing data confirmed precise cloning of intron at the desired position using cyclic ligation assembly. Successful expression of red fluorescence after cloning of intron confirmed successful intron recognition and splicing by host cell line. Cloning of intron increased the number of cells expressing red fluorescent protein. Conclusion. Cloning of intronic sequence within DsRed2 has helped to increase the number of cells expressing red fluorescence by approximately four percent.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers

ISSN

1213-8118

e-ISSN

—

Svazek periodika

161

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

354-359

Kód UT WoS článku

000418005200004

EID výsledku v databázi Scopus

2-s2.0-85038372050