Amniotic fluid cell-free DNA in preterm prelabor rupture of membranes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F18%3A10384913" target="_blank" >RIV/00216208:11150/18:10384913 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00179906:_____/18:10384913

Výsledek na webu

<a href="https://doi.org/10.1002/pd.5366" target="_blank" >https://doi.org/10.1002/pd.5366</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/pd.5366" target="_blank" >10.1002/pd.5366</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Amniotic fluid cell-free DNA in preterm prelabor rupture of membranes

Popis výsledku v původním jazyce

We evaluated the levels of cell-free nuclear DNA (nDNA) and cell-free mitochondrial DNA (mtDNA) in the amniotic fluid supernatant from pregnancies complicated by preterm prelabor rupture of membranes (PPROM) based on evidence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). A total of 155 women with PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. The levels of cell-free nDNA and mtDNA in the amniotic fluid supernatant were assessed and quantified by real-time polymerase chain reaction. The levels of cell-free nDNA and mtDNA were higher in women with MIAC and IAI than in women without these conditions (nDNA: with MIAC: median 3.9 x 10(4) genome equivalent [GE]/mL vs without MIAC: median 1.2 x 10(4) GE/mL, with IAI: median: 5.3 x 10(4) GE/mL vs without IAI: median 1.2 x 10(4) GE/mL; mtDNA: with MIAC: median 9.2 x 10(5) GE/mL vs without MIAC: median 2.5 x 10(5) GE/mL, with IAI: median 1.1 x 10(6) GE/mL vs without IAI: median 2.5 x 10(5); all P values &lt;= 0.01). Women with the microbial-associated IAI showed the highest levels of cell-free nDNA and mtDNA. Cell-free nDNA and mtDNA are constituents of the amniotic fluid supernatant from PPROM pregnancies. Both cell-free nDNA and mtDNA are involved in the intra-amniotic inflammatory response in women with PPROM.

Název v anglickém jazyce

Amniotic fluid cell-free DNA in preterm prelabor rupture of membranes

Popis výsledku anglicky

We evaluated the levels of cell-free nuclear DNA (nDNA) and cell-free mitochondrial DNA (mtDNA) in the amniotic fluid supernatant from pregnancies complicated by preterm prelabor rupture of membranes (PPROM) based on evidence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). A total of 155 women with PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. The levels of cell-free nDNA and mtDNA in the amniotic fluid supernatant were assessed and quantified by real-time polymerase chain reaction. The levels of cell-free nDNA and mtDNA were higher in women with MIAC and IAI than in women without these conditions (nDNA: with MIAC: median 3.9 x 10(4) genome equivalent [GE]/mL vs without MIAC: median 1.2 x 10(4) GE/mL, with IAI: median: 5.3 x 10(4) GE/mL vs without IAI: median 1.2 x 10(4) GE/mL; mtDNA: with MIAC: median 9.2 x 10(5) GE/mL vs without MIAC: median 2.5 x 10(5) GE/mL, with IAI: median 1.1 x 10(6) GE/mL vs without IAI: median 2.5 x 10(5); all P values &lt;= 0.01). Women with the microbial-associated IAI showed the highest levels of cell-free nDNA and mtDNA. Cell-free nDNA and mtDNA are constituents of the amniotic fluid supernatant from PPROM pregnancies. Both cell-free nDNA and mtDNA are involved in the intra-amniotic inflammatory response in women with PPROM.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30214 - Obstetrics and gynaecology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Prenatal Diagnosis

ISSN

0197-3851

e-ISSN

—

Svazek periodika

38

Číslo periodika v rámci svazku

13

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1086-1095

Kód UT WoS článku

000453544900014

EID výsledku v databázi Scopus

2-s2.0-85055682636