Alkaloid Profiling of Hippeastrum Cultivars by GC-MS, Isolation of Amaryllidaceae Alkaloids and Evaluation of Their Cytotoxicity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F20%3A10413519" target="_blank" >RIV/00216208:11150/20:10413519 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/20:10413519

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ywO9.K8Msq" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ywO9.K8Msq</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.25135/rnp.147.19.06.1302" target="_blank" >10.25135/rnp.147.19.06.1302</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Alkaloid Profiling of Hippeastrum Cultivars by GC-MS, Isolation of Amaryllidaceae Alkaloids and Evaluation of Their Cytotoxicity

Popis výsledku v původním jazyce

Different species, hybrids and varieties of the genus Hippeastrum (Amaryllidaceae) are planted for ornamental purposes and at the same time they represent a rich source of interesting secondary metabolites called Amaryllidaceae alkaloids. These compounds display a wide spectrum of biological activities. In the current study, six Hippeastrum taxa were evaluated for their alkaloid profile by GC-MS. Using preparative TLC, five alkaloids were isolated in pure form, and three of them evaluated for cytotoxic and antiproliferative potencies on a panel of human cancer cells of different histotype (Jurkat, MOLT-4, A549, HT-29, PANC-1, A2780, HeLa, MCF-7 and SAOS-2). In parallel, normal MRC-5 human fibroblasts were employed to determine the compounds&apos; overall toxicity against non-cancer cells. The most intriguing cytotoxic profile was demonstrated by montanine (1) with IC50 values between 1.04 - 1.99 mu M.

Název v anglickém jazyce

Alkaloid Profiling of Hippeastrum Cultivars by GC-MS, Isolation of Amaryllidaceae Alkaloids and Evaluation of Their Cytotoxicity

Popis výsledku anglicky

Different species, hybrids and varieties of the genus Hippeastrum (Amaryllidaceae) are planted for ornamental purposes and at the same time they represent a rich source of interesting secondary metabolites called Amaryllidaceae alkaloids. These compounds display a wide spectrum of biological activities. In the current study, six Hippeastrum taxa were evaluated for their alkaloid profile by GC-MS. Using preparative TLC, five alkaloids were isolated in pure form, and three of them evaluated for cytotoxic and antiproliferative potencies on a panel of human cancer cells of different histotype (Jurkat, MOLT-4, A549, HT-29, PANC-1, A2780, HeLa, MCF-7 and SAOS-2). In parallel, normal MRC-5 human fibroblasts were employed to determine the compounds&apos; overall toxicity against non-cancer cells. The most intriguing cytotoxic profile was demonstrated by montanine (1) with IC50 values between 1.04 - 1.99 mu M.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/EF18_069%2F0010046" target="_blank" >EF18_069/0010046: Předaplikační výzkum inovativních léčiv a medicínských technologií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Records of Natural Products

ISSN

1307-6167

e-ISSN

—

Svazek periodika

14

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

TR - Turecká republika

Počet stran výsledku

6

Strana od-do

154-159

Kód UT WoS článku

000499103400009

EID výsledku v databázi Scopus

2-s2.0-85076599932