3-Quinuclidinyl benzilate (agent BZ) toxicokinetics in rats
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F21%3A10430279" target="_blank" >RIV/00216208:11150/21:10430279 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60162694:G44__/21:00557158 RIV/00216208:11160/21:10430279
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=YNmOcUa0ec" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=YNmOcUa0ec</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/bcpt.13627" target="_blank" >10.1111/bcpt.13627</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
3-Quinuclidinyl benzilate (agent BZ) toxicokinetics in rats
Popis výsledku v původním jazyce
3-Quinuclidinyl benzilate (BZ) ranks among incapacitating military warfare agents. It acts as a competitive inhibitor on muscarinic receptors leading to non-lethal mental impairment. The present study aimed to investigate toxicokinetics of BZ in rats. Moreover, BZ can be exploited to produce a pharmacological model of Alzheimer's disease; thus, this paper focuses mainly on the BZ distribution to the brain. Wistar rats were administered i.p. with BZ (2 and 10 mg/kg). The BZ concentration was determined using LC-MS/MS in plasma, urine, bile, brain, kidney and liver. The sample preparation was based on a solid phase extraction (liquids) or protein precipitation (organ homogenates). The plasma concentration peaked at 3 min (204.5 +/- 55.4 and 2185.5 +/- 465.4 ng/ml). The maximal concentration in the brain was reached several minutes later. Plasma elimination half-life was 67.9 +/- 3.4 in the 2 mg/kg group and 96.6 +/- 27.9 in the 10 mg/kg group. BZ concentrations remained steady in the brain, with slow elimination (t(1/2) 506.9 +/- 359.5 min). Agent BZ is excreted mainly via the urine. Steady BZ concentration in the brain could explain the previously published duration of the significant impairment in passive avoidance tasks in rats after an injection of BZ.
Název v anglickém jazyce
3-Quinuclidinyl benzilate (agent BZ) toxicokinetics in rats
Popis výsledku anglicky
3-Quinuclidinyl benzilate (BZ) ranks among incapacitating military warfare agents. It acts as a competitive inhibitor on muscarinic receptors leading to non-lethal mental impairment. The present study aimed to investigate toxicokinetics of BZ in rats. Moreover, BZ can be exploited to produce a pharmacological model of Alzheimer's disease; thus, this paper focuses mainly on the BZ distribution to the brain. Wistar rats were administered i.p. with BZ (2 and 10 mg/kg). The BZ concentration was determined using LC-MS/MS in plasma, urine, bile, brain, kidney and liver. The sample preparation was based on a solid phase extraction (liquids) or protein precipitation (organ homogenates). The plasma concentration peaked at 3 min (204.5 +/- 55.4 and 2185.5 +/- 465.4 ng/ml). The maximal concentration in the brain was reached several minutes later. Plasma elimination half-life was 67.9 +/- 3.4 in the 2 mg/kg group and 96.6 +/- 27.9 in the 10 mg/kg group. BZ concentrations remained steady in the brain, with slow elimination (t(1/2) 506.9 +/- 359.5 min). Agent BZ is excreted mainly via the urine. Steady BZ concentration in the brain could explain the previously published duration of the significant impairment in passive avoidance tasks in rats after an injection of BZ.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30104 - Pharmacology and pharmacy
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Basic & Clinical Pharmacology & Toxicology
ISSN
1742-7835
e-ISSN
—
Svazek periodika
129
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
10
Strana od-do
246-255
Kód UT WoS článku
000668041800001
EID výsledku v databázi Scopus
2-s2.0-85108981136