Optimisation of an HPLC method for the simultaneous determination of pyrantel pamoate, praziquantel, fenbendazole, oxfendazole and butylhydroxyanisole using a phenyl stationary phase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10124375" target="_blank" >RIV/00216208:11160/12:10124375 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.rsc.org/en/content/articlepdf/2012/ay/c2ay05847b" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2012/ay/c2ay05847b</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c2ay05847b" target="_blank" >10.1039/c2ay05847b</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimisation of an HPLC method for the simultaneous determination of pyrantel pamoate, praziquantel, fenbendazole, oxfendazole and butylhydroxyanisole using a phenyl stationary phase

Popis výsledku v původním jazyce

A novel and simple isocratic reversed phase HPLC method with UV and fluorimetric detection for the simultaneous determination of the active anthelmintic components pyrantel pamoate, praziquantel, fenbendazole, its degradation product oxfendazole, and theantioxidant butylhydroxyanisole (BHA) has been developed and validated. The chromatography was performed using a Phenomenex Luna 3 mm phenyl-hexyl column (150 x 3.0 mm), and the mobile phase was composed of 0.5% triethylamine at pH 9.0 and acetonitrile55 : 45 (v/v) at a flow rate of 1.0 ml min(-1). The UV detection was performed at 290 nm for pyrantel, oxfendazole and fenbendazole and at 220 nm for praziquantel. For BHA, fluorescence detection was used with excitation and emission wavelengths of 287 nm and 322 nm, respectively. The method was validated and was applied for the determination of active compounds in various dosage forms of veterinary formulations.

Název v anglickém jazyce

Optimisation of an HPLC method for the simultaneous determination of pyrantel pamoate, praziquantel, fenbendazole, oxfendazole and butylhydroxyanisole using a phenyl stationary phase

Popis výsledku anglicky

A novel and simple isocratic reversed phase HPLC method with UV and fluorimetric detection for the simultaneous determination of the active anthelmintic components pyrantel pamoate, praziquantel, fenbendazole, its degradation product oxfendazole, and theantioxidant butylhydroxyanisole (BHA) has been developed and validated. The chromatography was performed using a Phenomenex Luna 3 mm phenyl-hexyl column (150 x 3.0 mm), and the mobile phase was composed of 0.5% triethylamine at pH 9.0 and acetonitrile55 : 45 (v/v) at a flow rate of 1.0 ml min(-1). The UV detection was performed at 290 nm for pyrantel, oxfendazole and fenbendazole and at 220 nm for praziquantel. For BHA, fluorescence detection was used with excitation and emission wavelengths of 287 nm and 322 nm, respectively. The method was validated and was applied for the determination of active compounds in various dosage forms of veterinary formulations.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods

ISSN

1759-9660

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

1592-1597

Kód UT WoS článku

000304768600020

EID výsledku v databázi Scopus

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