Hydroxysteroid dehydrogenases (HSDs) in bacteria - A bioinformatic perspective

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10133992" target="_blank" >RIV/00216208:11160/12:10133992 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0960076011001610" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0960076011001610</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jsbmb.2011.08.002" target="_blank" >10.1016/j.jsbmb.2011.08.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hydroxysteroid dehydrogenases (HSDs) in bacteria - A bioinformatic perspective

Popis výsledku v původním jazyce

The aim of this study was the identification of classified functional HSDs and the bioinformatic annotation of these proteins in all complete sequenced bacterial genomes followed by a phylogenetic analysis. For the bioinformatic annotation we constructedspecific hidden Markov models in an iterative approach to provide a reliable identification for the specific catalytic groups of HSDs. Here, we show a detailed phylogenetic analysis of 3 alpha-, 7 alpha-, 12 alpha-HSDs and two further functional relatedenzymes (3-ketosteroid-Delta(1)-dehydrogenase, 3-ketosteroid-Delta(4)(5 alpha)-dehydrogenase) from the superfamily of SDRs. For some bacteria that have been previously reported to posses a specific HSD activity, we could annotate the corresponding HSD protein. The dominating phyla that were identified to express HSDs were that of Actinobacteria, Proteobacteria, and Firmicutes. Moreover, some evolutionarily more ancient microorganisms (e.g., Cyanobacteria and Euryachaeota) were found as

Název v anglickém jazyce

Hydroxysteroid dehydrogenases (HSDs) in bacteria - A bioinformatic perspective

Popis výsledku anglicky

The aim of this study was the identification of classified functional HSDs and the bioinformatic annotation of these proteins in all complete sequenced bacterial genomes followed by a phylogenetic analysis. For the bioinformatic annotation we constructedspecific hidden Markov models in an iterative approach to provide a reliable identification for the specific catalytic groups of HSDs. Here, we show a detailed phylogenetic analysis of 3 alpha-, 7 alpha-, 12 alpha-HSDs and two further functional relatedenzymes (3-ketosteroid-Delta(1)-dehydrogenase, 3-ketosteroid-Delta(4)(5 alpha)-dehydrogenase) from the superfamily of SDRs. For some bacteria that have been previously reported to posses a specific HSD activity, we could annotate the corresponding HSD protein. The dominating phyla that were identified to express HSDs were that of Actinobacteria, Proteobacteria, and Firmicutes. Moreover, some evolutionarily more ancient microorganisms (e.g., Cyanobacteria and Euryachaeota) were found as

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Steroid Biochemistry and Molecular Biology

ISSN

0960-0760

e-ISSN

—

Svazek periodika

129

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

31-46

Kód UT WoS článku

000302106600005

EID výsledku v databázi Scopus

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