HILIC UHPLC-MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10145994" target="_blank" >RIV/00216208:11160/13:10145994 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.future-science.com/doi/full/10.4155/bio.13.217" target="_blank" >http://www.future-science.com/doi/full/10.4155/bio.13.217</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4155/bio.13.217" target="_blank" >10.4155/bio.13.217</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
HILIC UHPLC-MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development
Popis výsledku v původním jazyce
Background: Matrix effects are considered to be a main obstacle of quantitative bioanalytical LC-MS/MS methods. Therefore it is often required to minimize them in order to increase method reliability. HILIC has been referenced as one of possible approaches. However, there is a lack of experimental evidence in scientific literature so far. Methodology: Matrix effects were evaluated using spiked serum samples after SPE and protein precipitation prior to UHPLC-ESI-MS/MS. Chromatography was performed in both HILIC and reversed-phase mode. The influence of the matrix effects on the signal response was assessed using a set of 34 compounds of pharmaceutical interest and post-extraction addition approach. Results: The advantages and drawbacks of the HILIC andreversed-phase chromatographic modes were compared and discussed in detail. Conclusion: HILIC demonstrated the potential to reduce the occurrence of matrix effects when a more thorough sample pretreatment procedure such as SPE was applied
Název v anglickém jazyce
HILIC UHPLC-MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development
Popis výsledku anglicky
Background: Matrix effects are considered to be a main obstacle of quantitative bioanalytical LC-MS/MS methods. Therefore it is often required to minimize them in order to increase method reliability. HILIC has been referenced as one of possible approaches. However, there is a lack of experimental evidence in scientific literature so far. Methodology: Matrix effects were evaluated using spiked serum samples after SPE and protein precipitation prior to UHPLC-ESI-MS/MS. Chromatography was performed in both HILIC and reversed-phase mode. The influence of the matrix effects on the signal response was assessed using a set of 34 compounds of pharmaceutical interest and post-extraction addition approach. Results: The advantages and drawbacks of the HILIC andreversed-phase chromatographic modes were compared and discussed in detail. Conclusion: HILIC demonstrated the potential to reduce the occurrence of matrix effects when a more thorough sample pretreatment procedure such as SPE was applied
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FR - Farmakologie a lékárnická chemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/EE2.3.30.0022" target="_blank" >EE2.3.30.0022: Podpora vytváření, rozvoje a mobility kvalitních výzkumně-vývojových týmů na Univerzitě Karlově</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Bioanalysis
ISSN
1757-6180
e-ISSN
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Svazek periodika
5
Číslo periodika v rámci svazku
19
Stát vydavatele periodika
PT - Portugalská republika
Počet stran výsledku
13
Strana od-do
2345-2357
Kód UT WoS článku
000324909800009
EID výsledku v databázi Scopus
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