Gold Sputtered Fiducial Markers for Combined Secondary Ion Mass Spectrometry and MALDI Imaging of Tissue Samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F14%3A10293797" target="_blank" >RIV/00216208:11160/14:10293797 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.acs.org/doi/full/10.1021/ac500308s" target="_blank" >http://pubs.acs.org/doi/full/10.1021/ac500308s</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/ac500308s" target="_blank" >10.1021/ac500308s</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gold Sputtered Fiducial Markers for Combined Secondary Ion Mass Spectrometry and MALDI Imaging of Tissue Samples

Popis výsledku v původním jazyce

Mass spectrometry imaging (MSI) is a label free technique capable of providing simultaneous identification and localization of biomolecules. A multimodal approach is required that allows for the study of the complexity of biological tissue samples to overcome the limitations of a single MSI technique. Secondary ion mass spectrometry (SIMS) allows for high spatial resolution imaging while matrix-assisted laser desorption (MALDI) offers a significantly wider mass range. The combination of coregistered SIMS and MALDI images results in detailed and unique biomolecular information. In this Technical Note, we describe how gold sputtered/implanted fiducial markers (FM) are created and can be used to ensure a proper overlay and coregistration of the two-dimensional images provided by the two MSI modalities.

Název v anglickém jazyce

Gold Sputtered Fiducial Markers for Combined Secondary Ion Mass Spectrometry and MALDI Imaging of Tissue Samples

Popis výsledku anglicky

Mass spectrometry imaging (MSI) is a label free technique capable of providing simultaneous identification and localization of biomolecules. A multimodal approach is required that allows for the study of the complexity of biological tissue samples to overcome the limitations of a single MSI technique. Secondary ion mass spectrometry (SIMS) allows for high spatial resolution imaging while matrix-assisted laser desorption (MALDI) offers a significantly wider mass range. The combination of coregistered SIMS and MALDI images results in detailed and unique biomolecular information. In this Technical Note, we describe how gold sputtered/implanted fiducial markers (FM) are created and can be used to ensure a proper overlay and coregistration of the two-dimensional images provided by the two MSI modalities.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Chemistry

ISSN

0003-2700

e-ISSN

—

Svazek periodika

86

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

6781-6785

Kód UT WoS článku

000339227400009

EID výsledku v databázi Scopus

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