Determination of fluoroquinolones in fishes using microwave-assisted extraction combined with ultra-high performance liquid chromatography and fluorescence detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10365270" target="_blank" >RIV/00216208:11160/17:10365270 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0889157516302289" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0889157516302289</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jfca.2016.12.012" target="_blank" >10.1016/j.jfca.2016.12.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of fluoroquinolones in fishes using microwave-assisted extraction combined with ultra-high performance liquid chromatography and fluorescence detection

Popis výsledku v původním jazyce

A new analytical methodology based on ultra-high performance liquid chromatography with fluorescence detection (UHPLC-FD) has been developed for the determination of five fluoroquinolone antibiotics (FQs) used in aquaculture (norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin and sarafloxacin) in fish muscles (e.g. gilthead seabream (Sparus aurata)). The extraction and determination process was carried out by combining microwave-assisted extraction (MAE) along with a clean-up and pre-concentration step with solid phase extraction (SPE) followed by UHPLC-FD detection. The effects of different variables on MAE-SPE were studied and optimized using an experimental design. Validation was made in accordance with Decision 2002/657/EC. CC alpha and CC beta were determined according to the maximum residue limit (MRL). The method detection limit (MDL) of the entire process ranged between 0.1 and 6.0 ng g(-1). The recoveries obtained at two spiked concentrations levels (25 and 250 ng g(-1)) were greater than 90%, and the relative standard deviation was less than 8.7%. The developed methodology was successfully applied for the evaluation of the presence of FQs in fish muscle samples from aquaculture farms bought in various commercial shops in Gran Canaria Island (Spain).

Název v anglickém jazyce

Determination of fluoroquinolones in fishes using microwave-assisted extraction combined with ultra-high performance liquid chromatography and fluorescence detection

Popis výsledku anglicky

A new analytical methodology based on ultra-high performance liquid chromatography with fluorescence detection (UHPLC-FD) has been developed for the determination of five fluoroquinolone antibiotics (FQs) used in aquaculture (norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin and sarafloxacin) in fish muscles (e.g. gilthead seabream (Sparus aurata)). The extraction and determination process was carried out by combining microwave-assisted extraction (MAE) along with a clean-up and pre-concentration step with solid phase extraction (SPE) followed by UHPLC-FD detection. The effects of different variables on MAE-SPE were studied and optimized using an experimental design. Validation was made in accordance with Decision 2002/657/EC. CC alpha and CC beta were determined according to the maximum residue limit (MRL). The method detection limit (MDL) of the entire process ranged between 0.1 and 6.0 ng g(-1). The recoveries obtained at two spiked concentrations levels (25 and 250 ng g(-1)) were greater than 90%, and the relative standard deviation was less than 8.7%. The developed methodology was successfully applied for the evaluation of the presence of FQs in fish muscle samples from aquaculture farms bought in various commercial shops in Gran Canaria Island (Spain).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/EE2.3.30.0061" target="_blank" >EE2.3.30.0061: Zvýšení kapacity vědecko-výzkumných týmů Univerzity Karlovy prostřednictvím nových pozic pro absolventy doktorandských studií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Composition and Analysis

ISSN

0889-1575

e-ISSN

—

Svazek periodika

56

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

140-146

Kód UT WoS článku

000393526600018

EID výsledku v databázi Scopus

2-s2.0-85006380190