Copper(II) Sulfate Stimulates Scopoletin Production in Cell Suspension Cultures of Angelica archangelica

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10365366" target="_blank" >RIV/00216208:11160/17:10365366 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Copper(II) Sulfate Stimulates Scopoletin Production in Cell Suspension Cultures of Angelica archangelica

Popis výsledku v původním jazyce

Plant tissue cultures represent an attractive alternative source of valuable natural substances. Many strategies have been proposed for improving production yields, among others, elicitation with heavy metals. In this work, the effect was investigated of copper(II) sulfate (at concentrations of 0, 0.1, 0.5, 1, 5, 10, 50, 100, and 200 mu M) on scopoletin production, as well as on cell growth in Angelica archangelica (angelica) suspension cultures with the cultures maintained in the dark and in the light. Copper(II) sulfate up to 100 mu M did not significantly affect either fresh or dry biomass in the cultures grown in either the dark or light, but at 200 mu M they were slightly reduced. Copper(II) sulfate stimulated accumulation of scopoletin in a dose-dependent manner both in the cells and in the culture medium. Moreover, concerning scopoletin production, the cultures showed different sensitivity to copper ions depending on light conditions. The highest yields of scopoletin were found at copper concentrations of 5-50 mu M and 50 mu M in the light- and dark-grown cultures, respectively. Copper(II) sulfate has proved to be a suitable elicitor for improvement of scopoletin accumulation in cell suspension cultures of A. archangelica.

Název v anglickém jazyce

Copper(II) Sulfate Stimulates Scopoletin Production in Cell Suspension Cultures of Angelica archangelica

Popis výsledku anglicky

Plant tissue cultures represent an attractive alternative source of valuable natural substances. Many strategies have been proposed for improving production yields, among others, elicitation with heavy metals. In this work, the effect was investigated of copper(II) sulfate (at concentrations of 0, 0.1, 0.5, 1, 5, 10, 50, 100, and 200 mu M) on scopoletin production, as well as on cell growth in Angelica archangelica (angelica) suspension cultures with the cultures maintained in the dark and in the light. Copper(II) sulfate up to 100 mu M did not significantly affect either fresh or dry biomass in the cultures grown in either the dark or light, but at 200 mu M they were slightly reduced. Copper(II) sulfate stimulated accumulation of scopoletin in a dose-dependent manner both in the cells and in the culture medium. Moreover, concerning scopoletin production, the cultures showed different sensitivity to copper ions depending on light conditions. The highest yields of scopoletin were found at copper concentrations of 5-50 mu M and 50 mu M in the light- and dark-grown cultures, respectively. Copper(II) sulfate has proved to be a suitable elicitor for improvement of scopoletin accumulation in cell suspension cultures of A. archangelica.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Natural Product Communications

ISSN

1934-578X

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

2

Strana od-do

1779-1780

Kód UT WoS článku

000417552000032

EID výsledku v databázi Scopus

2-s2.0-85035243120