Probing the role of ceramide hydroxylation in skin barrier lipid models by H-2 solid-state NMR spectroscopy and X-ray powder diffraction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10382595" target="_blank" >RIV/00216208:11160/18:10382595 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0005273618300440" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0005273618300440</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbamem.2018.02.003" target="_blank" >10.1016/j.bbamem.2018.02.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Probing the role of ceramide hydroxylation in skin barrier lipid models by H-2 solid-state NMR spectroscopy and X-ray powder diffraction

Popis výsledku v původním jazyce

this work, we studied model stratum corneum lipid mixtures composed of the hydroxylated skin ceramides Nlignoceroyl 6-hydroxysphingosine (Cer[NH]) and alpha-hydroxylignoceroyl phytosphingosine (Cer[AP]). Two model skin lipid mixtures of the composition Cer[NH] or Cer[AP], N-lignoceroyl sphingosine (Cer[NS]), lignoceric acid (C24:0) and cholesterol in a 0.5:0.5:1:1 molar ratio were compared. Model membranes were investigated by differential scanning calorimetry and H-2 solid-state NMR spectroscopy at temperatures from 25 degrees C to 80 degrees C. Each component of the model mixture was specifically deuterated for selective detection by 2H NMR. Thus, the exact phase composition of the mixture at varying temperatures could be quantified. Moreover, using X-ray powder diffraction we investigated the lamellar phase formation. From the solid-state NMR and DSC studies, we found that both hydroxylated Cer[NH] and Cer[AP] exhibit a similar phase behavior. At physiological skin temperature of 32 degrees C, the lipids form a crystalline (orthorhombic) phase. With increasing temperature, most of the lipids become fluid and form a liquid-crystalline phase, which converts to the isotropic phase at higher temperatures (65-80 degrees C). Interestingly, lignoceric acid in the Cer[NH]-containing mixture has a tendency to form two types of fluid phases at 65 degrees C. This tendency was also observed in Cer[AP]-containing membranes at 80 degrees C. While Cer[AP]-containing lipid models formed a short periodicity phase featuring a repeat spacing of d = 5.4 nm, in the Cer[NH]-based model skin lipid membranes, the formation of unusual long periodicity phase with a repeat spacing of d = 10.7 nm was observed.

Název v anglickém jazyce

Probing the role of ceramide hydroxylation in skin barrier lipid models by H-2 solid-state NMR spectroscopy and X-ray powder diffraction

Popis výsledku anglicky

this work, we studied model stratum corneum lipid mixtures composed of the hydroxylated skin ceramides Nlignoceroyl 6-hydroxysphingosine (Cer[NH]) and alpha-hydroxylignoceroyl phytosphingosine (Cer[AP]). Two model skin lipid mixtures of the composition Cer[NH] or Cer[AP], N-lignoceroyl sphingosine (Cer[NS]), lignoceric acid (C24:0) and cholesterol in a 0.5:0.5:1:1 molar ratio were compared. Model membranes were investigated by differential scanning calorimetry and H-2 solid-state NMR spectroscopy at temperatures from 25 degrees C to 80 degrees C. Each component of the model mixture was specifically deuterated for selective detection by 2H NMR. Thus, the exact phase composition of the mixture at varying temperatures could be quantified. Moreover, using X-ray powder diffraction we investigated the lamellar phase formation. From the solid-state NMR and DSC studies, we found that both hydroxylated Cer[NH] and Cer[AP] exhibit a similar phase behavior. At physiological skin temperature of 32 degrees C, the lipids form a crystalline (orthorhombic) phase. With increasing temperature, most of the lipids become fluid and form a liquid-crystalline phase, which converts to the isotropic phase at higher temperatures (65-80 degrees C). Interestingly, lignoceric acid in the Cer[NH]-containing mixture has a tendency to form two types of fluid phases at 65 degrees C. This tendency was also observed in Cer[AP]-containing membranes at 80 degrees C. While Cer[AP]-containing lipid models formed a short periodicity phase featuring a repeat spacing of d = 5.4 nm, in the Cer[NH]-based model skin lipid membranes, the formation of unusual long periodicity phase with a repeat spacing of d = 10.7 nm was observed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/GC16-25687J" target="_blank" >GC16-25687J: Vztahy mezi zánětlivými procesy a bariérovými lipidy u onemocnění kůže</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica et Biophysica Acta - Biomembranes

ISSN

0005-2736

e-ISSN

—

Svazek periodika

1860

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

1162-1170

Kód UT WoS článku

000435057700024

EID výsledku v databázi Scopus

2-s2.0-85042066222